卡托普利对糖尿病肾病大鼠血浆同型半胱氨酸和肾功能的影响 .pdfVIP

卡托普利对糖尿病肾病大鼠血浆同型半胱氨酸和肾功能的影响

余娇;王繁麟;徐兵;童小文

【摘要】ObjectiveToinvestigatetheeffectofCaptoprilonplasmic

homocysteine(Hcy)andrenalfunctioninratswithdiabetic

nephropathy(DN)anditsmechanism.MethodsThediabetesmellitus

modelswereestablishedin30SpragueDawley(SD)ratswith

Streptozotocin(STZ),andwererandomlydividedintocontrolgroup,DN

groupandCaptoprilgroup(n=10foreachgroup).Captoprilgroupwas

lavagedwith17.5mg/(kg·d)Captopril(1/d),whilethecontrolandDN

groupswerelavagedwithsamevolumeofphysiologicalsaline(1/d).Inall

groups,contentsofplas-maHcyandangiotensinⅡ(AngⅡ)innephridial

tissue,levelsofureaandcreatinine,urinaryalbuminexcretionrate

(UAER)andkidneyweight/bodyweightweredetected,relativeexpression

levelsofmRNAinproteinkinaseC(PKC)andisoformsPKCαinnephridial

tissueweredetectedusingreal-timepolymerasechainreaction(PCR)after

12weeksoftreatment.ResultsComparedwiththoseincontrolgroup,the

valuesofHcyandAngⅡcontents,levelsofureaand

creatinine,UAER,kidneyweight/bodyweight,relativeexpressionsofmRNA

inPKCandPKCαweresignificantlyin-creasedinDNgroup(P﹤0.05).

ComparedwiththoseinDNgroup,theabovevaluesweresignificantly

decreasedinCaptoprilgroup(P﹤0.05).ConclusionCaptoprilmay

decreaselevelofHcyexpressionandprotectrenalfunctionofdiabetic

nephropathyrats,anditsmechanismmayexertitseffectbyinhibiting

PKCαsignalactivation.%目的：探讨卡托普利对糖尿病肾病（DN）大鼠血浆同

型半胱氨酸（homocystiene，Hcy）和肾功能的影响及其作用机制。方法采用链

脲佐菌素（strepotozotocin，STZ）诱导SD大鼠建立糖尿病模型30只，随机分

为空白对照组、DN组、卡托普利组3组，每组10只。卡托普利组以17.5mg/

（kg·d）卡托普利灌胃，空白对照组、DN组以等量生理盐水灌胃，1/d。于干预

后12周末检测各组大鼠血浆Hcy含量、肾组织血管紧张素Ⅱ（angiotensinⅡ，

AngⅡ）含量、尿素、肌酐、尿白蛋白排泄率（urinaryalbuminexcretionrate，

UAER）、肾重/体重，采用实时聚合酶链反应（real-timePCR）检测肾组织蛋白

激酶C（proteinkinaseC，PKC）及其亚型PKCα的mRNA相对表达水平。结

果与空白对照组比较，DN组Hcy含量、AngⅡ含量、尿素、肌酐、