拉喹莫德对中枢神经系统脱髓鞘小鼠胼胝体区少突胶质细胞的保护作用.pdfVIP

拉喹莫德对中枢神经系统脱髓鞘小鼠胼胝体区少突胶质细胞的

保护作用

刘思含;耿德勤;吴明凤;刘芷含

【摘要】目的探讨拉喹莫德(LAQ)对双环己酮草酰二腙(CPZ)诱导的C57BL/6脱

髓鞘小鼠胼胝体区少突胶质细胞的保护作用.方法将80只C57BL/6雄性小鼠随机

分为对照组(20只)、CPZ组(40只)、LAQ处理组(20只).免疫荧光法观察CPZ组

不同时间点(0d、3d、7d、2周、4周、8周)胼胝体区髓鞘碱性蛋白(MBP)和胶

质纤维酸性蛋白(GFAP)阳性细胞的分布,免疫印迹法检测三组各个时间点MBP和

GFAP蛋白水平,ELISA法检测TNF-α和IL-1β含量.结果对照组各个时间点均未见

脱髓鞘表现.与对照组相比,CPZ组MBP阳性细胞分布逐渐减少,然而GFAP阳性细

胞分布逐渐增多,8周形成胶质瘢痕.与CPZ组比较,LAQ组于4周、8周时MBP蛋

白表达均显著增加(均P0.05),于7d、2周、4周、8周时GFAP蛋白均显著降低

(均P0.05).与正常组相比,LAQ组和CPZ组于7d、2周、4周、8周时TNF-α、

IL-1β均显著升高(均P0.05).与CPZ组相比,LAQ组于2周、4周、8周时TNF-

α、IL-1β表达均显著减低(均P0.05).结论LAQ具有保护CPZ诱导的脱髓鞘小鼠

胼胝体区少突胶质细胞的作用,可能与抑制活化的星形胶质细胞分泌的炎症因子有

关.%ObjectiveToexploretheprotectiveeffectsoflaquinimod(LAQ)on

theoligodendrocytesatcorpuscallosuminC57BL/6micewithcuprizone

(CPZ)-induceddemyelination.MethodsEightyC57BL/6malemicewere

randomlydividedintocontrolgroup(n=20),CPZmodelgroup(n=40)and

LAQtreatmentgroup(n=20).Immunofluorescencestainingwasusedto

observethedistributionofmyelinbasicprotein(MBP)andglialfibrillary

acidicprotein(GFAP)positivecellsataseriesoftimepoints(0d,3d,7d,2

weeks,4weeks,8weeks)incorpuscallosum,theproteinexpressionsof

theMBPandGFAPinthethreegroupsweremeasuredbyWesternblotat

aseriesoftimepoints,thecontentsofTNF-αandIL-1βwereinvestigated

byELISA.ResultsThelossofmyelinsheathwerenotdetectedbyWestern

blotateachtimepointinthecontrolgroup.Comparewiththecontrol

group,MBP-positivecellsinCPZgroupshowedsparsedistribution

gradually.However,thedistributionofGFAP-positivecellsinLAQgroup

increasedgraduallyandthereappearedtobeglialscarat8

weeks.ComparedwithCPZgroup,theproteinexpressionofMBPwas

significantlyincreasedinLAQgroupat4,8weeks(allP0.05),butthe

proteinexpr