磷酸化蛋白质组学联合蛋白质组学分析敲除维甲酸诱导蛋白16对人结肠癌细胞的影响.pdfVIP

820肿瘤防治研究2024年第51卷第10期

Cancer

Res

Prev

Treat,2024,Vol.51,No.10

··

doi:10.3971/j.issn.1000-8578.2024.24.0351•基础研究•

磷酸化蛋白质组学联合蛋白质组学分析敲除

维甲酸诱导蛋白16对人结肠癌细胞的影响

12311

陈奕孛，苗根，王文，丁翠玲，戚中田

ProteomicsandPhosphoproteomicsAnalysisofEffectofRetinoicAcid-InducedProtein

16KnockoutonHumanColonCancerCells

12311

CHEN

Yibo,

MIAO

Gen,

WANG

Wen,

DING

Cuiling,

QI

Zhongtian

1.

Department

of

Medical

Biodefence,

Faculty

of

Naval

Medicine,

Naval

Medical

University,

Shanghai

200433,

China;

2.

Department

of

Nutrition

and

Food

Hygiene,

Faculty

of

Naval

Medicine,

Naval

Medical

University,

Shanghai

200433,

China;

3.

Tongji

University

School

of

Medicine,

Shanghai

200092,

China

Corresponding

Authors:

DING

Cuiling,

E-mail:

cuilingding@163.com;

QI

Zhongtian,

E-mail:

qizt@

Abstract:ObjectiveTo

analyze

the

differences

in

the

expressions

of

the

total

and

phosphorylated

proteins

in

human

colon

cancer

HCT116

cells

after

the

knockout

(KO)

of

retinoic

acid-induced

protein

16

(RAI16)

and

explore

the

possible

mechanism

and

related

signaling

pathways

affecting

its

protein

function

in

HCT116

cells.

MethodsHCT116

KO

and

WT

cell

proteins

were

collected

and

extracted,

and

the

protein

extraction

efficiency

was

detected

via

a

sodium

dodecyl

sulfate-polyacrylamide

gel

electrophoresis

(SDS)

experiment.

After

protein

digestion,

the

peptides

were

labeled

with

TMT

and

analyzed

via

mass

spectrometry.

We

used

bioinformatics

methods

to

analyze

the

identified

differential

proteins

and

differentially

phosphory-

lated

proteins

by

using

GO,

KEGG,

and

STRING

databases.

ResultsThe

results

of

SDS

showed

no

evident

protein

degradation.

In

addition,

some

key

bands

were

significantly

different

betw