胆固醇含量测定2011-10.pptVIP

Experiment

DETERMINATIONOF

SERUMTOTAL-ANDHDL-CHOLETEROL

第1页/共6页

principleLDLVLDLHDLmagnesiumphosphotungstate(磷钨酸镁)LDLVLDLSerumcholesterolfreecholesterolcholesterolesterRO以血浆脂蛋白形式运输HDL保留在上清中第2页/共6页

cholesterolesteraseCholesterolester+H2Ofreecholesterol+fattyacidcholesteroloxidaseFreecholesterol+O2?4-cholestenone+H2O22H2O2+4-aminoantipyrine+phenolredcompoundperoxidase+4H2OEnzymereagent:4-aminoantipyrine4-氨基安替比林phenol,peroxidase第3页/共6页

TubeH:0.2mlserum+0.2mlprecipitantTubeSH:0.4mlstandardcholesterol+0.4mlprecipitantStandfor15min2000rpm.10minsupernatantProcedure:第4页/共6页

unitmlTubeNO.TSTHSHBSupernatant----0.20.2--Serum0.02--------Cholesterolstandard--0.1------Distilledwater0.180.1----0.2Enzymereagent2.02.02.02.02.0Mix.Incubateat37℃.20min,Readtheabsorbanceat520nm,Baszero第5页/共6页

谢谢大家！ThankYou！