生物化学课件--专题讲座---A Brief Introduction of O.pptVIP

ABriefIntroductionofOligo?第一军医大学分子生物学研究所

一、GeneralInformation★Oligo?isamult-functionalsoftware。Itcanselecttheoligonucleotides(oligos)inageneortargetDNAsequence:1、Asprimers：usedforPCR，DNAsequencing，sitedirectedmutagenesis；2、Asprobes：usedforallkindsofDNAorRNAhybridizations。★Onlyafewcommercialsoftwarecanperformoligoanalysisandevaluation,whileOligo?isthebestamongthem。Itcancalculateaoligo’sTmbasedonthenearestneighbormethodandanalyzeallpossibleduplexesexistinthetargetoligo.★TheOLIGOsoftwarehasbeenbroadlya