外源基因GFP在白三叶草生物反应器系统中转录的研究.pptVIP

LeafAreafreshweightRT-PCR,Real-timePCRChlorophyll,totalRNA,proteinWesternBlotDataanalysisPlantSamples实验设计外源GFP瞬时表达的定量分析两个基因型叶片中叶绿素含量无显著差异Chlorophyll(ug)Chlorophyll(ug)030060090012001500Geno4Geno10Genotype不同发育阶段叶片中叶绿素含量变化Geno4Geno10Chlorophyll(ug)0200400600800100012003591113Leafnumberaaaaaab0200400600800100012003591113Leafnumberabccab重组蛋白产率平均水平：0.40%ofTSP（可溶性蛋白）重组人血清白蛋白 0.020%ofTSPHumanproteinC 0.001%ofTSPErythropoietin 0.003%ofTSP干扰素B 0.001%ofTSP例外：植物叶绿体表达系统中10%，36%，