核酸分子杂交.ppt

1.Basicprincipleofnucleicacidhybridization

2.Basicmethodofnucleicacidhybridization

3.BiochipTechnology

4.Preparationlabelofnucleicacidprobes

5.Factorsofnucleicacidhybridization;核酸分子杂交技术的发展史;TheNobelPrizeinPhysiologyorMedicine1993.

fortheirdiscoveriesofsplitgenes;DNA;Ovalbumingene;SectionI;denaturation;DNA变性是指在物理或化学因素的作用下，DNA分子互补碱基对之间的氢键断裂，使双链DNA分子变成两条单链DNA的过程。;1.FactorsthatcauseDNAdenaturation;2.changesinphysicalandchemicalpropertiesofdenaturedDNA;λ（nm）;OD260的应用;