苦菜的组织培养与快速繁殖.pdfVIP

( ) 安徽农业科学 ,Journal of Anhui Agri. Sci. 2005 ,33 2 :241 - 242 　　　　　　　　　　　　　　　　　　　　　责任编辑　金琼琼　责任校对　金琼琼 苦菜的组织培养与快速繁殖 ( ) 邱奉同 , 张渝洁 , 张 利　 临沂师范学院生命科学学院,山东临沂276005 摘要　通过对苦菜的组织培养 ,研究了外植体不同取材部位及不同激素种类、浓度对愈伤组织及芽诱导和增殖的影响。结果表明:苦 菜根的繁殖能力很强 ,但分化时间稍长;2 ,4D 对愈伤组织的诱导能力较强 ,NAA 对芽的诱导能力较强。同时 ,试验还筛选出了诱导愈 伤组织的适宜培养基:MS + 6BA 2. 0 mg/ L + 2 ,4D 0. 2 mg/ L ; 芽分化培养基:MS + 6BA 2. 0 mg/ L + NAA 0. 05 mg/ L ; 继代培养基:MS + 6BA 2. 0 mg/ L + NAA 0. 2 mg/ L 。 关键词　苦菜;组织培养;快速繁殖 ( ) 中图分类号　S636. 9 　　文献标识码　A 　　文章编号　0517 - 6611 2005 02 - 0241 - 02 Tissue Culture and Rapid Propagation of Ixeris Chinensis ( ) QIU Fengtong et al 　Life Science Department , Linyi Teachers ’University , Linyi ,Shandong 276005 Abstract 　The influence of different parts , different hormones and their concentrations on Ixeris Chinensi tissue culture inducing callus formation and bud growth was studied. The results showed that : during Ixeris Chinensis tissue culture , the root was easy to be induced , but it took more time to form bud. Through studying different hormones inducing buds and callus of Ixeris Chinensis ,we drew that : 2 ,4D induced callus more easily and NAA induced buds more easily. Simultaneously , in the experiment the appropriate media for the callus inducing was MS + 6 BA 2. 0 mg/ L + 2 ,4D 0. 2 mg/ L ,buds differentiation and subculture had been picked :MS + 6BA 2. 0 mg/ L + NAA 0. 05 mg/ L ,MS + 6BA 2. 0 mg/ L + NAA 0. 2 mg/ L. Key words 　Ixeris Chinensis ; Tissue culture ;Rapid propagation ( ) 2 　　苦菜[ Ixeris Chinensis Thunb. Nakai ] 是菊科苦菜属 1 年 1 cm 大小 ,分别接种到经高压灭菌的不同激素配比的诱导 生或多年生草本植物 ,别名清苦菜、苦荬菜。随着苦菜应用