原核生物eno 基因在系统进化中应用及水平转移分析.pdf

HEREDITAS (Beijing) 2012 7 , 34(7): 907―918 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2012.00907 原核生物eno 基因在系统进化中应用及水平转移分析 1 1 2,3 刘庆辉 , 郭振国 , 任嘉红 1. , 071002; 2. , 046011; 3. , 210037 多基因系统发育研究方法是系统发育分析中的一个重要手段, 基因树冲突已成为分子系统发育研究中 日益突出的问题。烯醇化酶基因(eno)及其编码的蛋白广泛存在于五界系统中, 烯醇化酶为糖酵解途径中重要酶 类。文章选取原核生物已注释的eno 基因序列进行了系统发育分析。对其中的 138 个模式菌株的eno 基因序列 进行系统发育分析和同源性搜索, 发现 19 个模式菌株的eno 基因是通过水平转移而来; 并通过核苷酸组成、密 码子偏好性和基因排列等基因特征分析, 进一步验证了水平转移基因的外源性。结果表明：原核生物eno 序列 具有较高保守性, 其大小适中, 是研究原核生物系统发育的良好材料。文章在对基因水平转移的供体和受体菌 株生活习性、进化历史以及烯醇化酶的结构和功能的研究过程中提供重要参考价值。 eno 基因; 系统发育; 基因水平转移 Phylogenetic application and analysis of horizontal transfer based on the prokaryote eno gene 1 1 2,3 LIU Qing-Hui , GUO Zhen-Guo , REN Jia-Hong 1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. Department of Biological Science and Technology, Changzhi College, Changzhi 046011, China; 3. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China Abstract: The phenomenon of conflicting gene trees has become a remarkable and difficult problem. Application of multiple genes has been a widespread practice to reconstruct phylogenies in phylogenetic studies. Enolase is a key glyco- lytic enzyme, The enzymes from a large variety of organisms, including archaebacteria, eubacteria and eukaryotes, were studied. We downloaded eno sequences from the genomes of bacteria and archaea that have been completely sequenced. The comprehensive homology search and phylogenetic analysis of the eno were used, and nineteen horizontally transferred genes were id