超声辐照破坏不同微泡对大鼠心肌组织SDF-1表达的影响.docVIP

  超声辐照破坏不同微泡对大鼠心肌组织 SDF-1 表达的影响# 罗丹，沈祥波，童嘉毅，陈龙** 5 10 15 20 25 30 35 40 （东南大学附属中大医院心内科，南京 210009） 摘要：背景：目前已有报道证实超声联合微泡能增效干细胞移植治疗心肌梗死，其机制可能 与超声微泡介导的心肌组织局部基质衍生因子-1（SDF-1）表达增加有关。目的：一氧化氮 （NO）微泡是在普通微泡的基础上加以改进后的新型微泡，本研究旨在探讨超声辐照破坏 不同微泡后大鼠心肌组织局部 SDF-1 的表达变化。方法：将 32 只 SPF 级 SD 雄性大鼠随机 分为 4 组，每组 8 只。第一、二、三组分别经尾静脉输入 1ml 的一氧化氮（NO）微泡（微 泡浓度为 1*108 个/ml）、普通微泡（微泡浓度为 1*108 个/ml）以及生理盐水，第四组为空 白对照组。输入完成后，第一、二、三组均采用频率为 1MHz，强度为 1W/cm2 的超声辐照 大鼠心前区，辐照时间为 10 分钟，第四组不做任何处理。1 周后，处死所有大鼠，取心肌 组织进行 RT-PCR 和 Western blot 分别检测 SDF-1 的基因及蛋白相对表达量。结果：RT-PCR 显示第一至第四组 SDF-1 相对表达量分别为 0.70±0.018、0.65±0.026、0.23±0.024 和 0.02 ±0.010。Western blot 显示第一至第四组 SDF-1 相对表达量分别为 0.89±0.024、0.78±0.017、 0.49±0.024 和 0.22±0.022。各组间进行两两比较，差异均有统计学意义（P0.05）。结论： 超声联合 NO 微泡辐照大鼠心肌组织，能使大鼠心肌组织 SDF-1 表达增加，并且其增加的 程度高于超声联合普通微泡。 关键词：心血管病学；超声；微泡；一氧化氮；SDF-1 中图分类号：R541.4 Effects of Ultrasound combined with different microbubbles on the expression of SDF-1 in rat Myocardium LUO Dan, SHEN Xiangbo, TONG Jiayi, CHEN Long (Department of cardiology,Zhongda Hospital, Southeast University, Nanjing 210009.) Abstract: Background: Ultrasound combined with microbubbles was proved to be effective in promoting stem cells homing,it may related with the homing factor Stromal cell-derived factor-1 (SDF-1). Objective:To investigate the quantity of SDF-1 expression after the intervention by different microbubbles on rats. Method: Thirty-two rats were divided into four groups randomly.Different kinds of microbubbles were given to group one,group two and group three through tail vein,group four was set for contrast . Ultrasound(1MHz,1W/cm2) was used to irradiate on rat precordium for ten minutes. Myocardium was harvested a week later for RT-PCR and Western blot to test the quantity of SDF-1 expression. Result:RT-PCR showed that the quantity of SDF-1 were 0.70±0.018, 0.65±0.026, 0.23±0.024 and 0.02±0.010.Western blot showed that the quantity of SDF-1 were 0.89±0.024, 0.78±0.017, 0.49±0.024 and 0.22±0.022. Compared with group two,group three and group f