基因芯片分析水稻灌浆期籽粒淀粉合成相关基因的表达.docVIP

  基因芯片分析水稻灌浆期籽粒淀粉合成相 关基因的表达 朱国辉，蔡珊兰* 5 10 15 20 25 30 35 40 45 （华南农业大学生命科学学院，广州 510642） 摘要：利用 Affymetrix 水稻基因芯片研究了水稻强势粒和弱势粒灌浆 3 d 和 9 d 的基因表达 变化，并着重分析了淀粉合成代谢相关基因的表达模式。结果表明，在检测到信号的 59 个 基因中，灌浆后 9 d 相比 3 d 表达丰度上调 2 倍的基因有 21 个，包括 SUS2、AGPS2、AGPL2、 GBSS、SSa、BE等，这类基因对水稻籽粒淀粉合成和灌浆起了至关重要的作用。同 时，此类基因在强势粒中的表达丰度以及上升幅度均高于弱势粒，表明强、弱势籽粒灌浆差 异可能源于淀粉合成能力的差异。灌浆后 9 d 相比 3 d 表达丰度下调 2 倍的基因有 12 个，包 括 CIN2、SPS3、AGPL1、GBSS、SSb 等，这类基因可能参与胚乳细胞基本结构的形 成以及初始淀粉粒的产生。 关键词：水稻；灌浆；淀粉合成基因；基因芯片 中图分类号：S51 Comprehensive Expression Analysis of Starch Biosynthesis Related Genes in the Developing Rice Grains Using DNA Microarrays ZHU Guohui, CAI Shanlan (College of Life Sciences, South China Agricultural University, GuangZhou 510642) Abstract: Rice grain ?lling is a process of starch accumulation. Biosynthesis of starch from sucrose in the developing endosperm primarily determines grain yield and quality of rice. DNA microarray analysis was performed in this study to investigate the differential expressed genes between 3 days and 9 days post anthesis in rice superior and inferior spikelets. 59 starch biosynthesis related genes were screened and among these, 21 genes were 2-folds up-regulated at 9 DPA (days post anthesis) compared with 3 DPA, including SUS2, AGPS2, AGPL2, GBSSⅠ, SSⅡa, BEⅠ, which may be the key genes responsible for the starch biosynthesis during grain filling. Meanwhile, these genes have higher mRNA profiles and increased multiple in superior spikelets than inferior spikelets, indicating that the difference of grain filling between superior and inferior spikelets may owe to, at least in part, their different starch biosynthesizing capacity, which was resulted by differetial expressions of starch bioynthesis genes. 12 genes were down-regulated at 9 DPA compared with 3 DPA, including CIN2, SPS3, AGPL1, GBSSⅡI, SSⅢb, which were presumed to be involved in the construction of fundamental cell machinery and initation of starch granules. Keywords: Rice (Oryza sativa L.); grain filling; starch b