青翘和老翘多种成分含量的比较.docVIP

  青翘和老翘多种成分含量的比较# 叶良红，龚小红，李芸霞** （成都中医药大学药学院，中药材标准化教育部重点实验室，中药资源系统研究与开发利用 5 10 15 20 25 30 35 40 省部共建国家重点实验室培育基地，成都 611130） 摘要：目的: 测定并比较青翘与老翘中连翘酯苷，连翘苷和连翘脂素的含量。方法: 以 HPLC 法测定青翘与老翘中连翘酯苷，连翘苷和连翘脂素的含量。连翘酯苷，连翘苷和连翘脂素分 别在 3.65~116.67μg/mL，3.65~116.67μg/mL 和 0.31~9.89μg/mL 范围内线性良好。R 均大于 0.999，RSD 均≥ 90%。结果: 青翘中的连翘酯苷为老翘的 10.67 倍，连翘苷相近，连翘脂素 的 1/2 倍. 结论：在治疗疾病的过程中，应该根据疾病的不同和青翘、老翘所含成分含量的 差别选择用药。 关键词：青翘；老翘；HPLC；连翘酯苷；连翘苷；连翘脂素 中图分类号：917 Compared the contents of mutiple components derived from qingqiao with laoqiao Ye Lianghong, Gong Xiaohong, Li Yunxia (Pharmacy College，Chengdu University of Traditional Chinese Medicine The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, ChengDu 611130) Abstract: Objective: To Compared the contents of multiple componengts derived from qingqiao to laoqiao. Method: The contents of multiple components were measured by HPLC. The good linear rangs of forsythoside A , phillyrin and phillygenin were 3.65～116.67μg, r=0.9999 and 0.31～ 10μg , r=0.9999 and 0.31～ 9.89μg. The values of RSD were greater than 90%. Result: Forsythiaside A contained in qingqiao is 10.67 times than laoqiao. Phillyrin of two individuls were near equal. RSD of precision and repeatability test were more than 90%. Conclusion: In the treatment of diseases, it is better to choice of medicine based on different symptoms of different illnesses associate with the contents of multiple componengts contained in qingqiao and laoqiao. Keywords: Qingqiao; Laoqiao; HPLC; Forsythiaside A; Phillyrin; Phillygenin 0 引言 连翘为木犀科连翘属植物连翘的干燥果实。依据其入药部位采收期和成熟度的不同又分 为两种规格。秋季果实初熟尚带绿色时采收入药习称青翘，果实熟透时采收入药习称老翘。 连翘是我国传统特色中药之一。早在《本草图经》中就有记载。连翘可以清热解毒，消肿散 结。具有抗炎［1-2］、抗病毒［3］、抗氧化［4-5］、抗过敏[6]和抗癌等活性［7］。临床 上治疗颈部肿瘤、食道癌、胃癌、乳癌、宫颈癌等多种癌症的配方中多以连翘为主要。更有 相关的研究报道连翘提取物具有抗癌活性，而其抗癌活性的物质基础就是连翘脂素［8］。 药典只规定了连翘酯苷和连翘苷含量测定方法，而连翘脂素则作为连翘的主要成分而非指标 成分纳入 2010 版药典，因此还没有明确的测定方法。本次研究通过建立一种简单、可行、 专