FoxE1基因启动子甲基化在结直肠癌和正常结直肠粘膜中的比较研究.docVIP

  FoxE1 基因启动子甲基化在结直肠癌和正 常结直肠粘膜中的比较研究# 张升1,3，龙亚东1,3，王志敏2，徐烨1,3，王海丰2，蔡三军1,3，蔡国响1,3** 5 10 15 20 25 30 35 40 （1. 复旦大学附属肿瘤医院大肠外科，上海 200032； 2. 国家人类基因组南方研究中心，上海 201203； 3. 复旦大学上海医学院肿瘤学系，上海 200032） 摘要：目的：探讨 FoxE1 基因启动子甲基化和结直肠癌发生的关系。方法：对 19 例 I 期结 直肠癌组织及其配对的正常结直肠粘膜上皮组织，采用实时定量甲基化 PCR 进行 FOXE1 基因 的甲基化检测，比较结直肠癌和正常结直肠粘膜上皮的 FOXE1 甲基化水平差异，探讨 FOXE1 基因甲基化和患者临床病理特征的关系。结果：FOXE1 甲基化阳性率为 63.2%（12/19），配 对的正常结直肠粘膜上皮组织均为非甲基化（0/19），结直肠癌的 FOXE1 甲基化水平显著高 于正常粘膜上皮组织（p＝0.029）。患者性别、年龄、肿瘤部位、组织学类型、分化程度、 浸润深度、脉管侵犯均和 FOXE1 基因启动子甲基化状态没有显著性关系。结论：FOXE1 启动 子甲基化具有癌特异性，在结直肠癌中的甲基化水平显著高于正常结直肠粘膜上皮组织。 关键词：结直肠癌；甲基化；FoxE1 中图分类号：R735.3 Comparative Study on Methylation of FOXE1 Gene Promotor between Normal Colorectal Mucosa and Colorectal Cancer ZHANG Sheng1,3, LONG Yadong1,3, WANG Zhimin2, XU Ye1,3, WANG Haifeng2, CAI Sanjun1,3, CAI Guoxiang1,3 (1. Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, ShangHai 200032; 2. Chinese National Human Genome Center at Shanghai, ShangHai 210203; 3. Department of Oncology, Shanghai Medical College, Fudan University, ShangHai 200032) Abstract: Objective:To explore the relationship between methylation of FOXE1 gene promotor and tumorigenesis of colorectal cancer.Methods: This study assessed the methylation of FOXE1 on 19 specimen of stage I colorectal cancer and 19 paired specimen of normal colorectal mucosa using realtime quantitative methylation-specific PCR, compared the difference on methylation of FoxE1 between normal colorectal mucosa and colorectal cancer, and explored the relationship between methylation of FOXE1 gene promotor and the clinicopathological features of colorectal cancer.Results: The positive rate of FOXE1 methylation is 63.2%（12/19）in stage I colorectal cancer, while none of the samples from normal colorectal mucosa was positive. The level of FOXE1 methylation of stage I colorectal cancer is significantly higher than that of normal colorectal mucosa (p=0.029). There are no significant correlation between the status of the FOX