3-羟基丁酸对体外培养的神经干细胞增殖及分化的影响.docVIP

  3-羟基丁酸对体外培养的神经干细胞增殖及 分化的影响# 杨志倩1，吕海侠2，焦倩2，李明川1，王媛媛2，冯敏娟2，卢晓云1** 5 （1. 西安交通大学生命学院，西安 2. 西安交通大学医学院，西安 710049； 710049） 摘要：目的：研究体外条件下 3-羟基丁酸（3-HB）对神经干细胞（NSCs）增殖及分化的影 响。方法：分离孕 14.5 天大鼠胚胎大脑皮质 NSCs，进行原代培养；培养至第三代的 NSCs 10 15 20 25 30 35 40 分别用 0.1、0.05、0.02、0.01g/l 3-HB 处理，未加药组作为对照，CCK-8 试剂盒检测细胞增 殖、活力，免疫细胞荧光化学观察 NSCs 分化情况。结果：与对照组相比，3-HB 处理对 NSCs 的增殖、活力没有显著影响；3-HB 处理组β-tubulin 阳性细胞（神经元）比例增加，GFAP 阳性细胞（胶质细胞）比例没有显著变化。结论：3-HB 对分离培养的 NSCs 在体外的增殖 和活力无明显影响，但能够促进其向神经元方向分化。 关键词：3-羟基丁酸；神经干细胞；细胞增殖；细胞分化 中图分类号：Q28 The effects of 3-hydroxybutyrate on the proliferation and differentiation of neural stem cells in vitro YANG Zhiqian1, LV Haixia2, JIAO Qian2, LI Mingchuan1, WANG Yuanyuan2, FENG Minjuan2, LU Xiaoyun1 (1. School of life science and technology, Xian Jiaotong University, Xian 710049; 2. Medical School, Xian Jiaotong University, Xian 710049) Abstract: Objective: Learn the effect of 3-hydroxybutyrate(3-HB) on the viability and differentiation of neural stem cell in vitro. Method: NSCs were isolated from the cerebral cortex of rat embryos on embryonic day 14-15, and P3 cells were used; NSCs were treated with different concentration(0.1、0.05、0.02、0.01g/l) of 3-HB, untreated as control. Cell viability was detected by CCK-8, and immune cell fluorescence chemistry was used to study the differentiation of NSCs. Result: Cell viability had no significant difference between 3-HB treated group and control. The percentage of β-tubulin positive cells was increased, but the percentage of GFAP positive cells had no dramatically alteration. Conclusion: Different concentration of 3-HB had no significant influence on NSCs viability, but could promote NSCs differentiate into neurons. Key words: 3-HB; neural stem cell; cell proliferation; cell differentiation 0 引言 随着人口老龄化，全球各种原因造成的脑损伤发病率呈现快速增长趋势，在我国，每年 仅新发脑卒中病例超过 200 万，死亡约 150 万，病后伤残率高达 28.7%（全球平均水平为 25%），严重影响国民健康和生活质量。由此引发的离婚率和失业率的增加、以及治疗脑损 伤的经济负担等已成为影响社会稳定的潜在因素。因此脑损伤后修复，尤其是神经功能重建 已成为医学和社会共同关注的问题，也是医学科学发展的终极挑战，是一项伟大的任务。 基金项目：教育部博士点新教师基金(2