生脉散抑制中性粒细胞呼吸爆发的物质基础研究.docVIP

  生脉散抑制中性粒细胞呼吸爆发的物质 基础研究# 吴雷，朱丹妮，余伯阳** 5 10 15 20 25 30 35 40 （中国药科大学中药复方研究室，南京 211198） 摘要：目的：利用中性粒细胞呼吸爆发为模型，考察生脉散对细胞释放氧自由基作用，追踪 其在有机体系下抗氧化的物质基础。方法：将生脉散水提物分成乙醚和正丁醇两个萃取部位， 以释放的氧自由基作为检测指标，测定生脉散抑制中性粒细胞呼吸爆发的主要活性部位，并 制备缺失其中某单味药的样品液进行活性考察，以确定各单味药对生脉散抑制中性粒细胞呼 吸爆发的贡献。结果：正丁醇部位是生脉散抑制中性粒细胞呼吸爆发的主要活性部位；麦冬 和人参是生脉散抑制中性粒细胞呼吸爆发的主要药味；皂苷类成分是抑制中性粒细胞呼吸爆 发主要活性成分。结论：生脉散中抑制中性粒细胞呼吸爆发的成分为生脉散正丁醇位中的皂 苷类化合物，中性粒细胞呼吸爆发为模型可用于抗氧化活性成分的筛选研究。 关键词：生脉散；抗氧化；皂苷 中图分类号：R284.1 The Study on the material bases of SMS for inhibiting neutrophil respiratory burst Wu Lei, Zhu Danni, Yu Boyang (Department of Complex Prescription of TCM, China Pharmaceutical University, NanJing 211198) Abstract: We pay attention to the influence of different fractions on OFRs production by the inhibition test of neutrophils respiratory burst. It was found that n-butanol extract of SMS was the major active fraction to inhibit neutrophil respiratory burst. In order to investigate the contributions of the single herbs to SMS, we prepared the effluents, which were removed one single herb. The effluents collected were assayed for the inhibition activity. The results showed the highest contributions of Ophiopogon japonicus and higher contributions of Panax ginseng to SMS for inhibiting neutrophil respiratory burst. To investigate the active components, HPLC was employed for eluting peaks basing on the chemical fingerprint. Effluents were collected in which the peaks of Panax ginseng and Ophiopogon japonicus were removed. The effluents collected were assayed for the inhibition activity. Mathematical method was employed to calculate the contribution rate of active peaks. Thus, the active peaks and their position in fingerprint could be revealed and identified by HPLC–DAD–MS/MS based on their retention behaviors, UV absorptions and MS spectra comparing with reference standards. It was shown that all the ingredients were the saponins. The n-butanol fraction, which showed weak activity for scavenging oxygen free radicals in vitro, was reg