溃疡性结肠炎脾胃虚实证候核糖体蛋白基因表达研究.pdfVIP

20 11 5 31 5 C JITWM, M ay 20 11, V ol. 31, N o5 603 1, 2 1, 3 1 1 1 1 研究健康人溃疡性结肠炎 ( ulcerat ve co l t s, U C )脾虚证和湿热证患者核糖体蛋白 ( r bo som a l prote n, RP ) 基因表达差异情 , 为从蛋白合成角度理解脾为气血生化之源提供实验依据 制作RP 基因芯片, 对4 名健康人4例 UC 脾虚证和 4例 UC 湿热证患者结肠黏膜进行检测, 应用 BR BTOOL ( 39) 软件包进行数据分析, 对差异基因进行生物信息学分析 成功制作了包括 77条 RP 基因, 2条 RP 类似基因( RPL 26l ke1RPL 7l ke1)的低密度芯片U C( 脾虚证 + 湿热证) 患者与健康人之间 有 12条差异基因, 全部为下调基因; 湿热证与健康人之间有 19条差异基因, 全部呈下调趋势; 脾虚证与健康 人有 3条差异表达基因, 全部为下调基因; 脾虚证与湿热证有 6条差异表达基因, 全部为上调基因聚类分 析显示健康人与UC 患者样品可以根据本芯片的基因表达谱进行分类, 湿热证和脾虚证可以通过聚类分开 多个差异基因有共同转录调节因子 成功制作了 RP 基因芯片, U C 脾虚证和湿热证 RP 基因表达下 调; 健康人U C脾虚证和UC 湿热证都有各自相应的RP基因表达谱 脾虚证; 湿热证; 基因芯片; 核糖体蛋白; 溃疡性结肠炎 Study on R bi osomalProtein Gene Expressoi n in Patei ntsw ith Ulcerative Colitis of Piasthenic Syndrome and Pi sthenic Syndrome CHEN Yulon , CHENW eiwen, LIRuliu, et al Institute of Gastroenterology, Guang zhou University of TraditionalChineseM edicine, Guangzhou (510405) ABSTRACT Ob jective To study differentia l expressoi n profiles of rbi osoma l protein ( RP ) enes in healthy subjects and ulce rative co litis ( UC ) patei nts of Piasthenic syndrome ( PAS) and of dampnessheat syndrome ( DHS), thus providin expermi entalbases for Pi as the source of q i and bol od theory from the view of protein synthesis. M ethods RP enes arrays were made. The mucousmembrane of cool nwas detected in four UC patients of PAS ( UCPAS), fourUC patients of DHS ( UCDHS), and four hea lthy subjects ( N), and data analyzed usin BRBTOOL Software Packa e (39). Bioinformatics anayl ses were conducted in differentia l enes. R esults Lowdensity RP ene chips were successfully produced, includ in 77 RP enes and two RP like enes ( RPL26like1 and RPL7like1). There were twelve d ifferential enes between UC