兽医专业英语.pptVIP

Immunomodulatory effect of swine CCL20 chemokine in DNA vaccination against CSFV 郑仲华 微生物学 1.Abstract The objective of this work was to explore whether a plasmid expressing CCL20 chemokine could improve the immune response against CSFV in co-administration with a DNA vaccine expressing the E2 protein. The immunization of pigs with the DNA vaccine formulation,that contains swine CCL20 chemokine, resulted in the homogenous induction of detectable levels of CSFV antibodies at 36 days after the ?rst injection. 2.Introduction It is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils（中性白细胞）. CCL20 is implicated in the formation and function of mucosal lymphoid tissues（粘膜淋巴组织） via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells（上皮细胞） surrounding these tissues. CCL20 elicits its effects on its target cells by binding and activating the chemokine receptor CCR6. Classical swine fever virus is the causative agent（病原体） of classical swine fever (CSF), one of the most important swine infectious diseases. CSFV is an icosahedral(二十面的) RNA virus with (+) polarity（正极） and a lipid envelope（脂质包膜） that belongs to the Pestivirus Genus（瘟病毒属）of the Flaviviridae family（黄病毒科）. 3.Materials and methods 4.Results 5.Discussion DNA immunization can be a useful tool for understanding the intrinsic immunological mechanisms involved in protection against a given pathogen or to map immunogenic protective determinants. Illustrating the versatility of DNA immunization, plasmid-based cytokines（细胞因子）,chemokines（趋化因子）, co-stimulatory molecules and enhancers of Dendritic Cells (DC) recruitment have been used as adjuvants to potentiate protective immune responses against different pathogens. Taken into account the feasibility of DNA immunization and its protective capacity against CSFV，we determined to use this methodolo