耐低温菊粉酶产生菌的分离及发酵条件的优化研究.doc

中文题目：耐低温菊粉酶产生菌的分离及发酵条件优化研究 外文题目：ISOLATION OF LOW-TEMPERATURE-RESISTANT INULINASE PRODUCING STRAIN AND OPTIMIZATION OF FERMENTATION CONDITIONS 毕业设计（论文）共50页（其中：外文文献及译文23页）图纸共0张 完成日期 2012年６月 答辩日期 2012年6月 摘要 在如今物质丰富，人们注重营养保健的年代，菊粉酶作为一种能将菊粉降解为高纯度果糖或低聚果糖的酶制剂，其工业化大规模生产越来越受到科研界及商业界的重视。目前国外对微生物菊粉酶进行了许多研究，包括菌种的筛选、产酶条件及酶的性质的研究等，我国关于菊粉酶的研究目前还停滞在实验室阶段，由于酶的产量低、生产成本高还未进行工业化应用,因此进一步筛选性能优良的高产酶菌株仍然十分重要。 本研究从低温保存的腐烂菊芋上分离出耐低温菊粉酶产生菌，以DNS比色法从33株菌株中筛选出菊粉酶高产菌株即2号菌株，其酶活力为11.44U/mL；通过Plackett-Burmen试验从豆粕添加量，菊粉添加量，(NH4) 2SO4添加量，磷酸氢二钾添加量，吐温添加量，氯化镁添加量，初始pH值，氯化钙添加量中筛选出影响发酵过程中菊粉酶活力的主要因素，即为豆粕添加量、初始pH值及氯化钙添加量，再通过设计响应面试验探究其最优发酵条件为：豆粕添加量2.58%，菊粉添加量2%，(NH4)2SO4 0.5%，磷酸氢二钾0.5%，吐温0.000%，氯化镁 0.2%，初始pH值6.0，氯化钙添加量0.42%；得到的此最佳发酵条件下的酶活力为19.18U/mL。 关键词：菊粉酶；高产菌株；发酵条件优化 Abstract In the era of material abundance,people pay more attention to nutrition and health,inulinase as a Enzyme preparation which can degradate inulin to high purity fructose or low-molecular-weight fructos,it large-scale production attract more and more attention by the research community and the business community.At present foreign study so much on microbial inulinase , including strains isolation, fermentation conditions and the nature of the enzyme, our studies on inulinase is still stuck in the laboratory stage, due to the low yield of enzyme and high production costs,it not yet industrialized application. Therefore, further screening of excellent performance, high-yield enzyme strains are still very important. The study separation low-temperature-resistant inulinase producing strains from the decayed artichoke kept at low temperature ,screening high yield inulin enzyme strains from the 33 strains by the method of DNS colorimetric,and the result show that 2nd strain enzyme is the highest yield Inulin enzyme ,enzyme activity lead to 11.44U/mL;Screening the main factor that affect inulinase activity in the fermentation process by Plackett-Burmen test from soybean pulp addition,inulin addition,(NH4)2SO4 addition,K2HPO4 addi