P-15 作为新型生物材料在治疗椎体压缩骨折中的应用的实验研究.docVIP

张  郡  呼  春  刘钦毅  摘要 吉林大学第二医院疼痛科，长春，130041 P-15是15种普通氨基酸肽合成的，属于类似人I型胶原蛋白α链的细胞粘附领域材 料。这是目前一个组织正在生产并使用作为表层无机骨的材料（ABM/P15），在这种形 式实验该材料具有增强细胞黏附能力，促进细胞分化、迁移和细胞生存[1]。我们打算 在体外培养进一步研究新的细胞迁移试验和试管定量实验。我们还打算在小鼠模型体 内研究探讨P15对于血管生成，软骨内成骨和骨形成的影响机制。结论P15可以增强骨 细胞粘附、促进细胞分化、迁移及提高细胞存活率，重要的是可以促进骨再生。 关键词：P15；骨质疏松；压缩性骨折；微创 责任作者及联系方式：刘钦毅，E-mail: liuqinyi1@ P-15作为新型生物材料在治疗椎体压缩骨折 中的应用的实验研究 Experimental Research on the Application of P-15 in the Treatment of Osteoporosisvertebra Compression Fracture as a New Biological Material Jun Zhang, Chun Hu, Qin-yi Liu Department of Pain, The Second Hospital of Jilin University, Changchun, 130041 Abstract P-15 is the common name of a synthetic 15 amino acid peptide (GTPGPQGIAGQRGVV) which mimics the cell-binding domain of alpha-1 chain of human collagen type I protein. It is currently being used as a tissue engineered product coating the surface of anorganic bone material (ABM/ P15). In this form it has demonstrated the ability to enhance cells adhesion, promote cell differentiation, migration and cell survival. The goal of our previous work was to evaluate if modification of the titanium surface with covalently linked P15 would enhance osteoblastic adhesion, proliferation and maturation, and investigate the mechanism by which this occurs. We propose in vitro investigation into the effects of P15 on using a novel cell migration assay and a tube formation assay. We also propose in vivo study to investigate the use of P15 for the enhancement of angiogenesis, endochondral ossification and bone formation in a subcutaneous mouse model. We found that P15 can enhance osteoblasts adhesion, promote cell differentiation, migration and cell survival, the most important is that it can promote bone regeneration. Key Words: P-15; Osteoporosis; Compression fractures; Minimally invasive Corresponding Author: Qin-yi Liu, E-mail: liuqinyi1@  引言 伴随着人们生活质量的提高，人类的寿命也逐渐延长， 意外或者机能衰老导致的骨折、骨损伤等疾病也日益增多， 随着社会人口逐渐趋于老龄化,其日益成为人们关注的热点, 尤其是老年患者由于病理性或者骨质疏松引起的脊柱压缩性 骨折，更是引起老年