5L发酵罐产L_乳酸的优化研究.pdf

2009 年第10 期 中 国 酿 造 总第211 期 · · 研究报告 41 5L发酵罐产L-乳酸的优化研究 高年发，刘 冰，王 勇，张 健 （工业微生物教育部重点实验室，天津科技大学生物工程学院，天津 300457） 摘 要：对乳杆菌亚种TL-4 的5L罐发酵条件进行了研究。确定了以玉米浆10mL/L，麸皮20g/L为氮源的发酵培养基，通风12h（通风量 200L/h ，搅拌转速150r/min），发酵56h，以400g/L氢氧化钙为中和剂，产酸达150g/L，转化率达94.62%。并通过二次补糖的分批补料发 酵方式，有效地消除了底物抑制作用，使产酸达172.5g/L，转化率达96.27%。氮源成本投入为200元/t L-乳酸左右。 关 键 词：L-乳酸；Lactobacillus sp. TL-4；5L-发酵罐；通风；中和剂；成本；分批补料发酵 中图分类号：TQ921 文献标识码：A 文章编号：0254-5071 （2009）10-0041-03 Fermentation optimization of L-lactic acid production by 5-L bioreactor GAO Nianfa, LIU Bing, WANG Yong, ZHANG Jian （Key Laboratory of Industrial Microbiology ，Ministry of Education, College of Biotechnology ， Tianj in University of Science and Technology ，Tianj in 300457，China） Abstract: Fermentation conditions of Lactobacillus TL-4 in 5-L bioreactor was studied. The maximum lactic acid production (150g/L) and the highest sugar utilization (94.62%) were obtained at concentrations of corn syrup and bran were 10 ml/L and 20 g/L, respectively, with ventilation at 200L/h, 150r/min for 12 h and with neutralizer of 400g/L Ca (OH) . Inhibition of substance could be decreased by fed-bacth fermentation, which deduced 2 concentration of L-lactic acid at 172.5g/L and sugar utilization at 96.27%. Cost of nitrogen source for L-lactic acid was abo