基于近红外光谱技术的三种硬实种子无损鉴定.pdfVIP

第28 卷 增刊2 农 业 工 程 学 报 Vol.28 Supp.2 2012 年 10 月 Transactions of the Chinese Society of Agricultural Engineering Oct. 2012 237 Nondestructive identification of hard seeds of three legume plants using near infrared spectroscopy 1 1 1 Zhu Liwei , Huang Yanyan , Wang Qing , 1 1,2 1,※ Ma Hanxu , Sun Baoqi , Sun Qun (1. Department of Plant Genetics and Breeding , College of Agriculture and Biotechnology, China Agricultural University / Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193, China; 2. Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China) Abstract: To explore the applicability of the NIRS on nondestructive identification of hardseedness characteristics, in this study, near infrared spectroscopy (NIRS) models were established using near-infrared spectroscopy combined with Partial Least squares (DPLS) to investigate the hard seed characteristics of legume seeds, Codariocalyx motorius Glycine max Cassia tora L.and Sophora alopecuroides. 120 seeds of the three species were divided into two groups: calibration set (80 samples) and validation set (40 samples), which contained the same number of hard seeds and soft seeds. The influences of replicate times, spectral region and calibration samples on the discrimination rate were compared. The results showed that with 4 000-5 000 cm-1 of