泰山灰树花液体深层发酵培养基的优化及海藻糖的提取.pdfVIP

现代食品科技 Modern Food Science and Technology 2008, Vol.24, No.11 泰山灰树花液体深层发酵培养基的优化及 海藻糖的提取 李艳玲，苗苗，王健美，史仁玖，李恒 （泰山医学院生物科学系，山东 泰安 271016） 摘要：本文对泰山灰树花液体发酵培养基的配方进行了优化，并对灰树花中海藻糖的测定条件和提取工艺进行了初步研究。结 果表明，优化的发酵培养基组成为：马铃薯200 g/葡萄糖40 g ，麸皮25 g ，板栗壳 150 g，磷酸二氢钾3 g，硫酸镁 1.5 g，水 1 L，菌 n 丝体生物量为 15.1 g/L。用0.2%蒽酮-硫酸溶液（料液比 1:4 ）测定菌体中海藻糖含量的条件：吸收波长625 nm，加热时间2 min。海 c 藻糖提取的工艺参数是：冰水浴，0.5 mol/L 三氯乙酸 20 mL ，提取次数为 3 次，每次提取时间 20 min ，共从 1.0 g 湿菌丝中提取到 14.522 . mg 海藻糖。 关键词：灰树花；发酵培养基；蒽酮-硫酸法；海藻糖 m 中图分类号：TS201.3；文献标识码：A ；文章篇号:1673-9078(2008)11-1141-04 o c Optimization of Fermentation Culture Medium for Grifola frondosa d. and Extraction of Trehalose o LI Yan-ling, MIAO Miao,WANG Jian-mei, SHI Ren-jiu, LI Hen (Department of Biological Sciences, Taishan Medical College, Taian 271016, China) o f Abstract: The fermentation medium composition of Grifola frondosa was optimized, and the extraction of trehalose from Grifola frondosa was studied. The results showed that the optimum component of medium of Grifola frondosa was: potato 200 g, glucose 40 g, wheat bran 25 g, n Chinese chestnut shell 150 g, KH PO 3g, MgSO 1.5g and water 1 L, under which the optimum weight of dry fungi was 15.1 g/L. The best 2 4 4 r determination conditions of trehalose content by 0.2% anthrone-sulphuric acid colorimetri