无血清培养体系中CD3AK 细胞诱导HL-60 细胞凋亡的研究.pdfVIP

李光金等　无血清培养体系中 CD3AK 细胞诱导 HL60 细胞凋亡的研究　第 7 期 3·53 · 无血清培养体系中 CD3AK 细胞诱导 HL60 细胞凋亡的研究 李光金 　陈功星 　严维耀 　郑兆鑫 (复旦大学生命科学学院遗传工程国家重点实验室 ,上海 200433) 　　中国图书分类号 　R392 　　文献标识码 　A 　　文章编号 　1000484X( 2001) ( ) 摘 　要 　目的 :采用无血清培养体系 ,研究 CD3 单克隆抗体激活的杀伤 CD3AK 细胞诱导 HL60 细胞凋亡情况。方法 :用 血清代用品代替人血清培养 CD3AK细胞 ,将诱导生成的 CD3AK细胞与 HL60 细胞共同孵育后 ,通过观察细胞形态、琼脂糖凝 ( ) 胶电泳检测 DNA 条带及流式细胞仪 FCM 检测分析 HL60 细胞凋亡情况。结果 :与 CD3AK细胞孵育 7 h 后的 HL60 细胞出现 凋亡细胞形态特征 ,DNA 凝胶电泳出现典型梯状电泳带 ,FCM 检测凋亡细胞比例为 2459 % 。结论 :无血清培养条件下 CD3AK 细胞可诱导 HL60 细胞凋亡。 关键词 　无血清培养 　CD3 单克隆抗体 　细胞凋亡 　效应细胞 　粒酶 Study on HL60 cell apoptosis induced by CD3 McAbactivated killer cells in serum free culture system LI GuangJin , CHEN GongXing , YAN WeiYao et al . State Key Laboratory of Genetic Engineering , Fudan University , Shanghai 200433 ( ) Abstract 　Objective :To investigate whether CD3McAbactivated killer CD3AK cells could induce apoptosis in HL60 cells in serum free culture system. Methods :HL60 cells were coincubated with CD3AK cells expanded in serumfree medium ,then apoptosis was assessed by ( ) means of morphological studies ,DNAagarose gel electrophoresis and flow cytomether FCM analysis. Results :After 7 h of coincubation with CD3AK cells in serumfree medium ,HL60 cells showed morphological features of apoptosis and typical DNA ladder on gel electrophoresis. FCM analysis showed that 24. 59 % cells were found to undergo apoptosis. Conclusion :CD3AK cells can induce apoptosis in HL60 cells under serumfree culture conditions. Key words 　Serumfree culture 　AntiCD3 monoclonal antibody 　Apoptosis 　Granzyme ( )