纤维蛋白原对牙龈卟啉单胞菌黏附口腔上皮细胞的影响.pdfVIP

葛颂 吴亚菲 刘天佳 赵蕾 费晓露 ! ( Fg) 3 K , Fg, H (Pg ) K Pg Fg Fg; Fg , Fg, Pg , ! ; , ; ; Effect of fibrinogen on the adherence of P orp hy rom onas g ing iva lis to hum an oral epithelia l cells * * GE Song , W U Yaf ei, LI U T ianj ia, ZH A O L e i, FE I X iaolu D ep ar men of P er iodon o logy, Wes Ch ina School of S om a ology, S ichuan Univ ersi y, Chengdu 610041, Ch ina Corresp onding au hor: W U Yaf ei, Em ail: yaf eiw u@ om com, T el: 028 Abstract! Objective To study the role of fibrinogen molecule in the pathogenesis of periodontal 3 diseases M ethods An in vitro cell culture model w as used M ethyl H Thym idine radiolabeled P orp hyrom onas g ing ivalis (Pg ) ATCC 33277 were exam ined for their ability to adhere to and invade the confluentmonolayers of human oral epithelial K cells w ith or w ithout exogenous human fibrinogens by scintillation spectrometry R esults The addition of exogenous fibrinogensmade more amount of and higher ratios of adhesive and invasivePg, in contrast to the group w ithout exogenous fibrinogen (P 0001) A t different concentrations of exogenous fibrinogen, the amount and ratios of adhesive and invasive Pg varied significantly (P ∀ 0007) The higher concentrations of exogenous fibrinogenw as added, the greater amount and ratios of adhesive and invasivePg were foundConclusions F ibrinogen promotes the adherence ofPg to human oral epithelial cells and may play an important role in the pathogenesis of periodontaldiseases Key words! Fibrinogen; Porphyromonas gingivalis; acterial adhesion; Periodontics , , Fg, ,