基于全基因组的芽孢杆菌平均核苷酸同源性(ANI) 分析.docVIP

基于全基因组的芽孢杆菌平均核苷酸同源性（ANI）分析 刘波*胡桂萍 唐唯其 福建省农业科学院农业生物资源研究所，福建省福州市，350003 摘要 采用Jspecies软件分析芽孢杆菌全基因组之间平均核苷酸同源性（ANI）的特征性。结果表明，芽孢杆菌属种间两两菌株间ANI具有明显属种特异性，其中芽孢杆菌科不同属之间的ANI值50%-65%；芽孢杆菌ANI值均低于95%，到%，加权平均数为70.12%；芽孢杆菌亚种ANI值分布范围%到%，。同时基因组中的四核苷酸（Tetra）回归系数与ANI值具有相关性，表现明显种特征性，回归拟合显示两者呈现一元二次方程关系，（R2=0.9812），同时高于70的ANI值与相应的Tetra回归系数呈线性正相关，方程式为（R2=0.8767）。 关键词 芽孢杆菌，系统发育，平均核苷酸同源性，Jspecies Characteristic of average nucleotide identity based on the whole genomes from Bacillus species in Bacillus-like genus Liu Bo*, Hu Gui-ping, Tang Wei-qi Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China Abstract: The characteristic of average nucleotide identity（ANI）based on the whole genome from Bacillus species in Bacillus-like genus was analyzed using the soft of Jspecies. The results showed that ANI distribution appeared the species specificity and was correlated with the relationship of taxonomic and evolutionary affinities. The ANI values computed from different Bacillus-like genus were below a 65% identity or above a 50% different in orthologous genes identity. The ANI values from different Bacillus species were below 94% with a wide range from 63.77 to 93.81 and the most of them ranging from 64.77% to 68.4% with the weighted average of 70.12%. The ANI value between subspecies was mainly ranged from 90% to 96%. So, the ANI criterion of the identification for genus, species and subspecies within Bacillus was suggested as 50-65%，65-90% and 90-96%, respectively. Furthermore, Tetra regression index was correlated with the ANI value and displayed the species specificity. Regression fitting analysis showed that the equation of y=271-573.56x+399.65x2 with the correlation coefficient of 0.9812 occurred between the Tetra and the ANI values above a identity of 70 were positive correlated with Tetra regression index with the equation of y=178.58x-81.521 and the correlation coefficient. of 0.8767. Key words: Bacillus, phylogeny, average nucleotide identity, J