双波长比色法测定马铃薯直链支链淀粉含量.pdfVIP

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.1 双波长比色法测定马铃薯直链/支链淀粉含量 曾凡逵，赵鑫，周添红，刘刚 （中国科学院兰州化学物理研究所环境材料与生态化学发展中心，甘肃兰州 730000） 摘要：将新鲜马铃薯中的淀粉提出后，采用双波长比色法测定了直链/支链淀粉含量。淀粉经I -KI 溶液染色，直链淀粉呈蓝色， 2 支链淀粉呈紫色。535 和 570 nm 用于直链淀粉含量测定，555 和 760 nm 用于支链淀粉含量测定。结果表明，样品溶解是检测的关键， 淀粉与碘试剂的反应时间会对测定结果造成一定影响。同时通过本方法的检测，新大坪品种马铃薯的直链淀粉含量为21.20% ，支链 淀粉含量为 78.80%。 关键词：双波长比色法；直链淀粉；支链淀粉；马铃薯 文章篇号：1673-9078(2012)1-119-122 Dual-wavelength Colorimetric Method for Measuring Amylose and Amylopectin Contents of Potato Starch ZENG Fan-kui, ZHAO Xin, ZHOU Tian-hong, LIU Gang (Center for Eco-Materials and Eco-Chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China) Abstract: Dual-wavelength colorimetry was developed to determine amylose and amylopectin contents after the starch was isolated from potato. By staining with I2-KI solution, amylose displayed a blue color, amylopectin displays a violet color. Absorbancies at 535 and 570nm were used for amylose content measurement, absorbancies at 555 and 760 nm are used for amylopectin content measurement. The XIN DAPING potato starch (from Dingxi, Gansu province) contained 21.20 % of amylase and 78.80 % of amylopectin. The detection results can be affected by the sample dissolution and the reaction time of starch and I2-KI solution. Key words: dual-wavelength colorimetry; amylose; amylopectin; potato 直链淀粉、支链淀粉含量的测定方法包括差示扫 Hovenkampf Hermelink[8] 报道了双波长比色法 [1] 描量热仪法（DSC ），高效空间排阻色谱法（HPSEC ） （618 和550 nm）测定淀粉样品中直链淀粉的含量。 [2] [3] [4] Zhu[9]