HPLC-ICP-MS法分析测定药用植物中的形态汞.pdfVIP

《现代仪器与医疗》 2013 年 第 19 卷 第 2 期 研究报告 HPLC-ICP-MS法分析测定药用植物中的形态汞* Determination of mercury species in chinese herbs by HPLC-ICP-MS 摘  要  确定针对连翘、红花、番泻叶、西洋参、川穹、当归等药用植物中的汞形态（甲 基汞、乙基汞、二价汞离子）超声提取样品前处理方法，采用phenomenex C18 液相色 1  2 2 宋 旭   孔维恒 **  高 峰   谱柱进行分离，ICP-MS 检测。样品回收率72.83%~98.25% ，精密度（RSD ）(n=6) 均 2 3 2 李 晶   朱平丽   王金花 1 2 小于15%，甲基汞、乙基汞、二价汞的检出限分别为0.125mg/kg ，0.250mg/kg ，0.125 Song Xu Kong Weiheng 2 2 mg/kg 。该方法具有灵敏度高、干扰小、样品前处理简单等优点，可用于药用植物中 Gao Feng Li Jing Zhu Pingli3 Wang Jinhua 2 汞形态的筛查和测定。 关键词  高效液相色谱- 电感耦合等离子体质谱 汞形态分析 (1.北京市怀柔第一医院  北京  101400；2.北京出入境检验检 Abstract The pre-treatment method of ultrasonic for mercury speciation (Methylmercury, 疫局  北京  100026；3.珀金 Dimercurion , Ethylmercury) of forsythia, safflower, senna, gen-seng, Rhizoma Chuanxiong, 埃尔默仪器（上海）有限公司  angelica in Chinese herbs were established. Mercury speciation were separated using the 上海  201203） phenomenex C 18 column of high performance liquid chromatography with inductively (1.Huairou The First Hospital, Beijing coupled plasma mass spectrometry. The recoveries of three mercury speciation were 101400; 2.Technical Centre, Beijing 72.83%~98.25%. Relative standard deviations (n=6) were 15%. Limit of detections for Entry-Exit Bureau, Beijing 100026; Methylmercury, Dimercurion and Ethylmercury were 0.005mg/L, 0.010mg/L, 0.005mg/L 3.PerkinElmer Instruments(Shanghai) Co.,Ltd, Shanghai 201203) respectively. The advantages of the method were easy sample extra