微囊藻毒素粗提物对水环境中温和气单胞菌活的非可培养状态的影响.pdfVIP

J. Lake Sci.(湖泊科学) , 2008, 20(1): 105-109 . E-mail: jlakes@ ©2008 by Journal of Lake Sciences 微囊藻毒素粗提物对水环境中温和气单胞菌活的非可培养状态的影响* 潘高山, 胡章立** , 雷安平, 黎双飞 (深圳大学生命科学学院, 深圳大学生态环境研究所, 深圳 518060) 摘 要: 细菌 VBNC 状态(Viable but non-culturable state), 又称“活的非可培养状态”, 是自然水体中广泛存在的细菌的一种 特殊存活状态, 复杂的环境因子变化是 VBNC 状态转换的可能原因. 本研究利用接种温和气单胞菌的无菌湖水, 研究铜绿微 囊藻(Microcystis aeruginosa)培养液和微囊藻毒素粗提液对水环境中温和气单胞菌 VBNC 状态转换的影响, 结果发现, 温和气 单胞菌在无菌湖水中同时存在 VBNC 状态和可培养状态 ; 产毒微囊藻培养液和微囊藻毒素粗提液均能促进温和气单胞菌中 VBNC 细菌与可培养细菌之间的状态转换, 说明微囊藻毒素可能是水环境中影响细菌 VBNC 状态转换的重要环境因素, 其进 一步的分子机制研究正在进行中. 关键词: 微囊藻毒素; 温和气单胞菌; 细菌 VBNC 状态 Effect of crude microcystin on the viable but non-culturable state of Aeromonas sobria in aquatic enviroment PAN Gaoshan, HU Zhangli, LEI Anping LI Shuangfei ( College of Life Science, Shenzhen University, Institute of Ecology and Environment, Shenzhen 518060, P.R.China) Abstract: Viable But Non-Culturable (VBNC) is a special state of bacteria for survival. It is a conventional state for bacteria in natural water body. Complicated environment factors may be the rational reason of the interchange. In this study, the effects of cyanobacterial media and crude microcystin on the VBNC state of A. sobria were conducted by incubating Aeromonas sobria into a sterile lake water. The results showed that some bacteria were in the VBNC state while others in culturable state; both cyanobacterial media and crude microcystin could improve the change of A sobria from VBNC state to culturable state. It revealed that microcystin might be an important environmental factor involved in the VBNC state transfering in aquatic environment. More research concerned about molecular mechanism was undergoing. Keywords: Aeromonas sobria ; microcystin; bacterial VBNC state Colwell 等[1]首次发现了细菌的一种特殊状态, 即“活的非可培养(Viable but non-culturable state, VBNC)”状态. 在进一