13尼古丁降解菌L1的分离鉴定与降解特性分析.pdfVIP

∗ 13. 尼古丁降解菌 L1 的分离鉴定与降解特性分析 1 1 3 2 2 雷丽萍 ，夏振远 ，王玥 ，魏海雷 ，刘杏忠 ** (1.云南省烟草科学研究所，玉溪 653100；2.中国科学院微生物研究所真菌地衣系统学重点实验室，北京 100080；3.北京林业大学生物科学与技术学院，北京 100083) 摘要: 菌株L1分离自云南烟草叶片，能有效降解1.5 g/L的尼古丁。通过形态观察，16S rDNA序列分析和生 理生化测定将其鉴定为Bacillus simplex 。菌落生长密度测定和高压液相色谱分析表明， L1菌株最适生长 尼古丁浓度为1g/L，随着菌落密度增加，尼古丁降解效率逐渐升高，36 h达到最高降解率75.0%。而低尼古 丁含量不利于L1菌株的生长，过高尼古丁含量对L1菌株的生长和降解效率具有反馈抑制作用。菌株L1降解 尼古丁过程中无色素产生，说明其尼古丁代谢途径有别于节杆菌。 关键词: 尼古丁；芽孢杆菌；生物降解 中图分类号: 文献标识码: Isolation and Characterization of Nicotine-degrading Bacterial Strain L1 1 3 2 2 LEI Li-ping , XIA Zhen-yuan1, WANG Yue , WEI HAI –lei LIU Xing-zhong ** (1. Yunnan Tobacco Science Research Institute, Yuxi 653100; 2. Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080; 3. College of Biological Sciences and Biotechnology; Beijing Forestry University, Beijing 100083) Abstract: A bacterial strain L1, capable of degrading 1.5g/L nicotine, was isolated from tobacco leaves. It was identified as a member of Bacillus simplex based on morphology, physiological and chemical tests, 16S rDNA sequence and phylogenetic characteristics. Strain L1 could utilize nicotine as sole source of carbon and nitrogen and its optimal growth concentration of nicotine was 1.0g/L, while less and more nicotine concentration went against growth. 75.0% of nicotine could be degraded by strain L1 under the optimized incubation conditions for 36 h monitored by high-performance liquid chromatography. But no pigment was presented at degrading process. This study demonstrates that Bacillus simplex L1 had strong ability to degrade nicotine, and the degrading mechanism may be different from Arthrobacter sp. Key words: Nic