超临界CO2杀菌过程中萃取机制研究.pdfVIP

14 2010, Vol. 31, No. 17 食品科学 ※基础研究 CO2 1 , 2 2 1 2 2 2 周先汉 ，程丽梅 ，曾庆梅 ，张 安 ，宋俊骅 ，周典飞 (1.合肥工业大学 农产品生物化工教育部工程研究中心，安徽 合肥 230009 ； 2.合肥工业大学生物与食品工程学院，安徽 合肥 230009) 在相同工艺条件下对大肠杆菌菌悬液作超临界 CO2 处理，对不同时间段萃取液的成分进行分析。结果表 明，萃取物主要有两大类：非极性脂肪酸类及极性较弱的小分子氨基酸类物质，均为细胞的功能性物质。证实在 超临界 CO2 杀菌过程中确实发生了萃取作用。 超临界 C O 2 ；萃取；杀菌机理；大肠杆菌 Extraction Mechanism during the Sterilization of Supercritical Carbon Dioxide 1,2 2 1 2 2 2 ZHOU Xian-han ，CHENG Li-mei ，ZENG Qing-mei ，ZHANG An ，SONG Jun-hua ，ZHOU Dian-fei (1.Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China； 2.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China) Abstract：Functional components in cytoplasm, cell membrane and cell wall can be dissolved by supercritical carbon dioxide. As a result, the cells will be damaged and die due to inactivation. The extraction mechanism during supercritical carbon dioxide sterilization was explored in this study. An Escherichia coli suspension at a concentration of 108 CFU/mL was prepared and treated with supercritical carbon dioxide and composition analysis was performed on the extracts obtained at different time points. The results indicated that both non-polar fatty acids and polar weak small amino acids were the functional components in cells. This investigation demonstrates that extraction indeed occurs during supercritical carbon dioxide sterilization. Key words ：supercritical carbon dioxide ；extraction ；sterilization mechanism ；Escherich