微波消解石墨炉原子吸收法测定软胶囊壳中重金属铬的方法学研究.pdfVIP

网络出版时间：2012-04-28 15:47 网络出版地址：/kcms/detail/11.2162.R1547.001.html 微波消解-石墨炉原子吸收法测定软胶囊壳中重金属铬的方法学研究 * 喆 ，冯克然，张露勇，张玉婷，宋钰，曹进，张庆生，王钢力(中国食品药品检定研究院，北京 100050） 摘要：目的 建立微波消解-石墨炉原子吸收法测定软胶囊壳中铬的方法。方法 采用微波消解法对软胶囊壳样品进行消解， 在波长357.9 nm 下，通过石墨炉程序升温进行测定，狭缝宽度为 0.5 nm ，塞曼效应作为背景校正。结果 方法线性关系良 好，相关系数r=0.999 6；检出浓度为 0.6 mg ·L-1，以称样量0.5 g 计，方法检出浓度为0.06 mg ·kg-1；加标回收率为90.0 ～ 110%。结论 该方法简便，可行，适用于软胶囊壳中重金属铬的测定。 关键词：软胶囊壳；原子吸收光谱；铬 中图分类号：R917 文献标识码：A 文章编号：1001-2494(2012)09-0717-03 Determination of Chromium in Soft Gel by Microwave Digestion-Graphite Furnace Atomic Absorption Spectrophotometry DONG Zhe ，FENG Ke-ran, ZHANG Lu-yong, ZHANG Yu-ting, SONG Yu, CAO Jin, ZHANG Qing-sheng, * WANG Gang-li (National Institutesf or Food and Drug Control Beijing 100050 China) ABSTRACT OBJECTIVE To establish a method of microwave digestion-graphite furnace atomic absorption spectrophotometry for the determination of chromium in soft capsule shells. METHODS Microwave digestion was adopted to digest the soft capsule shells. Graphite furnace temperature control program was optimized ，Zeeman effect was used in the background correction，the slit width was 0.5 nm，and the content of chromium was determined under the wavelength of 357.9 nm ．RESULTS The calibration curve was linear in the range of 0-50 mg L-1, (r=0.999 6). The detection limit was 0.06 mg kg-1, and the average recoveries were 90%-110%. CONCLUSION This method is simple，accurate, and suitab le for the determination of chromium in soft capsule shells. KEY WORDS soft capsule shell; atomic absorption spectrophotometry; chromium 药用和食用明胶为动物胶原蛋白不完全酸水解 1.2 仪器与条件 (A 型)、碱水解(B 型)或酶降解后纯化得到的