脱水植物组织培养基的制备.pdfVIP

河　北　轻　化　工　学　院　学　报 第 19 卷　　第 2 期 . 19　 . 2 JOU RNAL O F H EBE I IN ST ITU T E O F CH EM ICAL V o l N o 总第 45 期　1998 年 T ECHNOLO GY AND L IGH T INDU STR Y Sum 45　1998 脱水植物组织培养基的制备 仪　宏　王丽丽　董秀平　高延庭 　温春秀 　及　华 河北科技大学生物科学与工程系, 石家庄　050018 　　摘　要: 脱水培养基可以在很大程度上简化植物组织培养基的配制工艺, 但由于 一般植物组培培养基成分之间可能相互反应、不同组分的含量相差很大导致难以混 合均匀等因素的制约, 脱水植物组织培养基的制备较困难。笔者提出并研究了喷雾浸 润分配工艺和糖包盐造粒隔离工艺, 成功地制备了可用于植物组织培养的脱水培养 基。 　　关键词: 植物组织培养; 脱水培养基; 制备 　　《中国图书资料分类法》分类号: S 336 T he P reparaion of D ry M edium U sed fo r P lan t T issue Cu ltu re 　 　 　 　 　 Y i Hong W ang L ili Dong X iup ing Gao Yan ting W en Chunx iu J i H ua , D epartm ent of B io logical Science Engineering H ebei U niversity of Science and T echno logy, Sh ijiazhuang, 050018 Abstract: U sing dry m edium w ill facilitate the p reparaion of p lant tissue culture m edium . greatly But usually it is difficult to p repare the dry m edium suitable fo r p lant tissue culture since there exist som e obstacles such as the po ssible chem ical reactions among ino rganic salts and the distribution difficulty due to the great ratio betw een the amount of trace elem ents and that of m ain . “ ” “ ” ingredients T he sp ray soak ing distribution m ethod and the