维拉帕米对腹腔镜胆囊切除术病人氧化应激反应的影响.pdfVIP

暨南大学硕士学位论文 摘 要 目的目的：评价术前静注维拉帕米对腹腔镜胆囊切除术病人氧化应激反应的影响。 目的目的 方法方法：患慢性胆囊炎合并胆囊结石炎症急性发作行择期腹腔镜胆囊切除术 （LC 方法方法 手术）的病人30 例，年龄 18-50 岁，ASA 分级 Ⅰ-Ⅱ，随机分为对照组（C 组） 和维拉帕米组 （V 组），每组15 例。除维拉帕米组于气腹前 10 分钟静脉注射 维拉帕米 5mg 外，两组麻醉、气腹和手术方式均相近。两组病人分别于手术前、 手术气腹结束即刻及术后 24 h 抽静脉血标本，测定血清酶ALT、AST 和脂质过 氧化代谢产物 MDA 浓度。结果结果：与术前比较，C 组和V 组之 ALT 和 AST 值在术 结果结果 后放气即刻及术后 24 小时两个时间点均有显著增高 （P0.05），其中 V 组增高 程度明显低于 C 组（P0.01）；V 组之 MDA 值在术后放气即刻及术后 24 小时两 个时间点较术前无明显增高（P＞0.05），两组比较，V组明显低于C组（P0.01）。 结论结论：术前静注抗氧化剂维拉帕米能有效减小气腹带来的氧化应激反应： ，有效 结论结论：： 保护肝脏等内脏功能。 关键词关键词：腹腔镜： ；氧化应激；维拉帕米 关键词关键词：： I 暨南大学硕士学位论文 Abstract Objective: To assess the protective effects of verapamil on oxidative stress injury in patients underwent laparoscopic cholecystectomy(LC). Methods: 30 patients (18-50yr of age, ASA physical status Ⅰor Ⅱ) with cholelithiasis and cholecystitis undergoing selective LC were randomly assigned to Control group(C) and Verapamil group(V), with 15 patients in each group. The patients in group C were just subjected to a usual operation; the patients in group V were allocated to inject 5mg verapamil 10 min before insufflation. Blood samples of every patient were taken pre-, postinsufflation and 24h postoperatively. The levels of blood alanine aminotransferase (ALT), aspartate aminotransferase (AST) and malondialdehyde (MDA) were measured. Results: there was a significant increase in liver enzymes （ALT and AST ） after surgery in both groups （P0.05）, but values of these two enzymes were significantly lower in group V than those in group C （P0.01）. Meanwhile, the increase of serum MDA in group V was less than