临床研究尿素通道蛋白在正常人和尿毒症患者汗腺细胞中的表达.pdfVIP

J South Med Univ, 2013, 33(7): 951-955 doi 10.3969/j.issn. 1673-4254.2013.07.03 ·951 · 临床研究 尿素通道蛋白在正常人和尿毒症患者汗腺细胞中的表达尿素通道蛋白在正常人和尿毒症患者汗腺细胞中的表达 1 2 2 刘 静 ，解立怡 ，尹爱萍 1 2 西安交通大学 医学院，第一附属医院肾脏内科，陕西 西安 710061 摘要：目的探讨尿素通道蛋白（UTs ）在正常人和尿毒症患者中皮肤及汗腺细胞表达情况。方法 切取尿毒症及肾功正常者腹部 皮肤及腋臭患者的大汗腺组织，采用免疫组化SP法及免疫荧光法检测汗腺组织中UTs 的表达特征，定量分析UTs 在尿毒症及 对照组间表达的差异。结果 UTs 在人皮肤基底层细胞及大、小汗腺组织中均有表达。N-UT-A 1、UT-B 1蛋白亚型在尿毒症组的 小汗腺细胞中表达高于对照组，差异有统计学意义（P0.05），C-UT-A1蛋白亚型在两组间的表达差异不大（P0.05）。结论 人 皮肤基底层细胞、小汗腺细胞及大汗腺细胞中均表达UTs ，且UTs 在尿毒症患者的小汗腺中表达比正常人更高。 关键词：尿素通道蛋白；汗腺细胞；尿素；尿毒症 Expression of urea transporters in sweat gland tissue of normal subjects and uremic patients 1 2 2 LIU Jing , XIE Liyi , YIN Aiping 1 2 Xian Jiaotong University College of Medicine , Department of Nephrology, First Affiliated Hospital , Xian Jiaotong University, Xian 710061, China Abstract: Objective To explore the expression of urea transporters (UTs) in the skin and sweat glands of normal subjects and patients with uremia. Methods Abdominal skin biopsy samples of patients with uremia and normal patients and apocrine sweat gland tissue from patients with bromhidrosis were examined for the expression of UTs using immunohistochemistry and fluorescence immunoassay for quantitative analysis. Results Both UT-A1 and UT-B1 proteins were expressed in the skin basal cell layer, eccrine sweat gland and apocrine sweat gland tissues. In uremic patients, N-UT-A1 and UT-B1 expressions were significantly higher than those in the control (P0.05) but C-UT-A1 expression was similar (P0.05). Conclusion UTs are expressed in human skin basal cell layer, eccrine sweat gland and apocrine sweat gland tissues, and