紫癜汤抑制ITP 模型小鼠血小板破坏的作用机制研究.docVIP

紫癜汤抑制ITP模型小鼠血小板破坏的作用机制研究 李杨，曲道炜，林庶如，郝宏党，范颖 （辽宁中医药大学，沈阳110032） 摘要：目的：紫癜汤对特发性血小板减少性紫癜 ( ITP) 模型小鼠脾中巨噬细胞（ＭΦ）凋亡及微观结构的影响，探讨该方临床使用的作用机制。方法：将48只 BALB/C 小鼠随机分为4组：正常组、模型组、醋酸泼尼松组、紫癜汤组，除正常组外，其余各组采用免疫法腹腔注射GP-APS( 豚鼠抗小鼠血小板血清) 建立 ITP 小鼠模型，观察小鼠外周血小板、脾中巨噬细胞的微观结构及其凋亡情况。结果：治疗后，两药物组均能提高ITP小鼠血小板计数，与用药前比较有显著性差异；紫癜汤组ＭΦ的凋亡率提高，与模型组比较P0.05，疗效优于醋酸泼尼松；两药均能改善ＭΦ的微观结构，紫癜汤优于醋酸泼尼松。结论：紫癜汤通过改善ITP 模型小鼠脾中巨噬细胞的微观结构及促进巨噬细胞的凋亡起到治疗作用。 关键词：紫癜汤；特发性血小板减少性紫癜；巨噬细胞；凋亡 Zhidian Decoction inhibiting ITP mouse model of platelet destruction mechanism research LI Yang,QU Dao-wei,LIN Shu-ru,HAO Hong-dang,FAN Ying (Liaoning University of Traditional Chinese Medicine,Shenyang,110032,China) [Abstract]Objective: To observe the effects of Zhidiantang impact on the ITP model mouse spleen macrophage apoptosis and microphage，to explore the mechanism of action of the clinical use of the prescription. Methods:The 48 BALB / C mice were randomly divided into 4 groups:the normal group，model group，prednisone acetate group，Zhidiantang group，ITP mouse model were made by Immunoassay intraperitoneal injection of GP-APS (guinea pig anti-mouse platelet serum). Observe of the mouse peripheral platelet and spleen macrophages microscopic structure and apoptosis. Results: After treatment，the treatment group can raise platelet count ,there are significant difference compared with before treatment; After treatment, MΦ apoptosis rate was improved in Zhidiantang roup compared with the model group, P 0.05 and is superior to prednisone acetate group. Conclusion: zhidiantang play a therapeutic effect by improving the microscopic structure of the the ITP model mouse spleen macrophages and promote macrophage apoptosis. [Key words] Zhidiantang; idiopathic thrombocytopenic purpura; macrophage; apoptosis 材料与方法 1材料 1.1实验动物 SPF级健康BALB/C小鼠，体重为18-22克，7-8周龄，48只，雌雄各半，由北京维通利华动物技术有限公司提供。 基金项目：辽宁省教育厅创新团队项目［2009T065］。 第一作者：李杨，女，副教授，博士学位，主要从事中药药效学研究。 通讯作者：范颖，教授，博士生导师，E-mail:lnzyfy@ 126.com 1.2药物 紫癜汤组成:黄芪25g、制大黄15g、人参10g、阿胶25g、桃仁10g、生地10g、白芍20g、仙鹤草15g、当归10g、甘草10