水稻植株及其内生菌内生微生物脂肪酸生物标记分布特性的研究.docVIP

水稻植株及其内生微生物脂肪酸生物标记分布特性的研究 胡桂萍，刘波*，朱育菁，郑雪芳，林营志，史怀 (福建省农业科学院农业生物资源研究所，福州市，350003) 摘要 以兴优制5、兴优HK02、准优HK05、准优航653和水稻组培苗为材料，采用改良脂肪酸法分析水稻植物自身脂肪酸和其内生微生物脂肪酸结构组成，结果检测到得到水稻自身和其内生微生物共有脂肪酸为12:0、16:0、18:0和20:0。水稻自身脂肪酸有直链脂肪酸20:0、12:0、16:0、18:0和不饱和脂肪酸如18:3ω6с(6,9,12)、19:0 CYCLOω8с、a17:0、i16:1 H、16:0 N ALCOHOL、15:1ω6с、i15:0 3OH。水稻内生微生物脂肪酸主要有支链脂肪酸，如甲基脂肪酸，羟基脂肪酸等，共有22种如i20:0、20:1 ω9с、12:0 3OH等。内生微生物脂肪酸在不同水稻品种含量差异很大，有完全分布和单一分布。对内生微生物脂肪酸进行判别分析得到，遗传距离近的水稻品种其判别距离也近。 关键词 水稻 组培苗 内生微生物 脂肪酸 Distribution characteristics of fatty acids contained in the plants from rice and their endophytes Gui-Ping Hu, Bo Liu*, Yu-Jing Zhu, Xue-Fang Zheng, Yin-Zhi Lin, Huai Shi （Agricultural Biological Resource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003） Abstract: Distribution characteristics of fatty acids contained in the plants from rice and their endophytes were analysised by modified method of fatty acids with four hybrid rice and regenerated plantlet combinations as materials. The four hybrid rice were xingyouzhi5、xingyouHK02、zhunyouHK05、zhunyouhang653. The results revealed that fatty acids obtained 12:0、16:0、18:0 and 20:0 were detected in plant and endophytes in common. And fatty acids of rice obtained Straight Chain Fatty Acid such as 20:0、12:0、16:0、18:0 and unsaturated fatty acids such as 18:3ω6с(6,9,12)、19:0 CYCLOω8с、a17:0、i16:1 H、16:0 N ALCOHOL、15:1ω6с、i15:0 3OH. 22 kinds of the fatty acids were detected in the endophyes of rice includeiso- and anteiso-methyl-branched fatty acids，hydroxy fatty acids and so on , for example i20:0、20:1 ω9с、12:0 3OH. The contents of the fatty acids from endophytes in different rice were different and they represented completely distrituion and singularity distrituion. The discriminant function ananlysis of the fatty acids from endophytes showed that distances of DF were relation with the distances of heredity in rice. Key words：Rice; Regenerated Plantlet; Endophytes; Fatty acids 前言 植物体和微生物体都含有丰富的脂肪酸。脂肪酸是生物体细胞组分中一种稳定而富有的重要成分，是构成生物膜的重要物质