CART肽减轻小鼠脑缺血再灌注急性脑水肿.docVIP

  CART 肽减轻小鼠脑缺血再灌注急性脑 水肿# 刘丽冰1，邱彬1，周彦君2，陈曼1，祝世功1** 5 10 15 20 25 30 35 40 （1. 北京大学医学部生理学与病理生理学系，北京 100191； 2. 潍坊医学院麻醉学系，山东 潍坊 261053） 摘要：目的：研究 CART 肽对脑缺血再灌注急性期脑水肿的作用及时间窗。方法：采用线 栓法复制雄性小鼠大脑中动脉阻塞（MCAO）和再灌注模型。实验分 Sham 组、MCAO 组、 四个 CART 治疗组（ 0h、2h、3h 和 4h 组）。通过 TTC 染色检测脑梗死体积及肿胀体积， 应用干湿重法测定脑水含量，伊文思蓝法检测血脑屏障（BBB）的损坏程度。结果：MCAO 组小鼠脑梗死体积、肿胀体积、脑水含量和伊文思蓝漏出率都明显高于 Sham 组，CART 0h 组的脑梗死体积、肿胀体积、脑水含量和伊文思蓝漏出率明显低于 MCAO 模型组。CART 2h、 3h 组脑肿胀体积明显低于 MCAO 组，CART 4h 组脑肿胀体积与 MCAO 组没有明显差异。 结论：（1）CART 肽能减轻脑缺血再灌注引起的脑肿胀和脑水含量，抑制血脑屏障的破坏； （2）再灌注后 0h 给予 CART 肽作用明显，2h 和 3h 给予 CART 肽仍然有效。 关键词：CART 肽；脑缺血；脑水肿；血脑屏障 中图分类号：R363 CART peptide reduces acute brain edema during ischemia/reperfusion in mice Liu Libing1, Qiu Bin1, Zhou Yanjun2, Chen Man1, Zhu Shigong1 (1. Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing 100191; 2. Department of Anaesthesiology, Weifang Medical University, ShanDong WeiFang 261053) Abstract: Objective：To explore the effect of CART peptide to brain edema in the acute phase of ischemia/reperfusion and the time-window. Methods：Brain infarct volume and swelling volume was measured by TTC staining. Using the wet-dry weighting method to measure the water content. The extent of blood–brain barrier （BBB） disruption was investigated using Evans blue extravasation. Results: After 22h reperfusion, CART peptide significantly reduced brain infarct volume and swelling volume. At the same time, water content in brain tissue was decreased by CART peptide treatment, and BBB disruption was limited with CART peptide than without. In the therapeutic time-window study, CART peptide, when administrated during 3 hours after reperfusion, can reduce brain infarct volume and swelling volume. Conclusion: CART peptide significantly reduces the brain edema and disruption of BBB when CART was injected at 0h, 2h and 3h after reperfusion. Keywords: CART peptide; cerebral ischemia; brain edema; blood–brain barrier 0 引言 可卡因-苯丙胺转录调节肽（cocaine- and amphetamine– regulate