AEG-1调控头颈鳞癌血管内皮生长因子表达的体外实验.docVIP

  AEG-1 调控头颈鳞癌血管内皮生长因子表 达的体外实验# 朱刚才，刘勇，余长云，李果，粟忠武，谭浩蕾，邱元正，张欣，田勇泉** 5 （中南大学湘雅医院耳鼻咽喉头颈外科，长沙 410008） 摘要：目的 体外实验中探讨星形胶质细胞上调基因 1 (AEG-1)对主要促血管生成因子血管 内皮生长因子（VEGF）的调控作用。方法 利用慢病毒介导的 AEG-1 过表达载体感染头颈 鳞癌 Tu686 细胞，Western blot 及酶联免疫吸附法（ELISA）分别检测 AEG-1 蛋白上调后 10 VEGF 蛋白的胞内表达及胞外分泌量的改变。结果 头颈鳞癌 Tu686 细胞 AEG-1 蛋白上调 后，Western blot 显示实验中 VEGF 蛋白胞内表达量较亲本细胞及对照组显著增高。ELISA 表明胞外分泌的 VEGF 蛋白在对照组和亲本细胞组中的表达分别为（343.3 ± 7.65）pg/ml 和（358.8 ± 12.03）pg/ml，而实验组中的表达显著升高为（480.9 ± 13.15）pg/ml，差异 具有统计学意义（F = 90.51, P 0.01）。结论 AEG-1 蛋白可促进头颈鳞癌细胞主要促血管 15 20 25 30 35 40 生成因子 VEGF 蛋白的表达。 关键词：头颈部肿瘤；癌，鳞状细胞癌；星形胶质细胞上调基因-1；血管内皮生长因子；转 移 中图分类号：R739.6 AEG-1 moderates the expression of VEGF in head and neck squamous cell carcinoma ZHU Gangcai, LIU Yong, YU Changyun, LI Guo, SU Zhongwu, TAN Haolei, QIU Yuanzheng, ZHANG Xin, TIAN Yongquan (Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha 410008) Abstract: Objective: To investigate that AEG-1 protein can promote regulate the expression of VEGF protein in squamous cell carcinoma of the head and neck (SCCHN) in vitro. Methods: SCCHN Tu686 cells were transfected with lentivirus mediated AEG-1 overexpression vector. Western blot was used to examine the expression of AEG-1 protein and cytoplasmic VEGF protein. Enzyme-linked immunosorbent assay （ELISA） was applied to assay the expression level of VEGF secretion caused by AEG-1 overexpression. Results: AEG-1 protein was successfully overexpressed in SCCHN Tu686 cells. Western blot results revealed that AEG-1 overexpression led to upregulation of cytoplasmic VEGF protein. ELISA data showed that the secretion of VEGF protein was significantly increased in Tu686 cells transfected with AEG-1 overexpression vector（480.9±13.15）pg/ml, when compared with Tu686 cells transfected with empty vector (343.3±7.65)pg/ml and parental Tu686 cells (358.8 ±12.03)pg/ml (F = 17.091, P 0.01). Conclusions: The present study demonstrated that AEG-1 protein can upregulate the