CART抑制OGD诱导培养大鼠皮层神经元内质网应激.docVIP

  CART 抑制 OGD 诱导培养大鼠皮层神经元 内质网应激# 邱彬1，贾增强2，刘丽冰2，王亚宸1，祝世功1** 5 10 15 20 25 30 35 （1. 北京大学医学部生理学与病理生理学系，北京，100191； 2. 山东省潍坊医学院，潍坊，261053） 摘要：目的：探讨可卡因-安非他命调节转录肽（CART）对糖氧剥夺（OGD）诱导的大鼠 皮层神经元内质网应激的作用。方法：18 天 SD 大鼠胚胎（E18）皮层神经元原代培养 8 天， 采用糖氧剥夺法（OGD）诱导神经元损伤。用 MTT 法检测细胞的存活率、western blotting 方法检测内质网应激反应标志分子（GRP78，caspase 12 和 CHOP）。结果：与对照组比较， OGD 组的神经元存活率显著降低（P 0.05），内质网应激标志分子 GRP78、CHOP 和 caspase12 表达增多（P 0.05）。CART 预处理组神经元的细胞活性明显高于 OGD 组（P 0.05），GRP78、CHOP 和 caspase12 表达明显低于 OGD 组（P 0.05）。结论：CART 通 过抑制内质网应激反应减轻 OGD 诱导的大鼠皮层神经元损伤。 关键词：神经保护；可卡因-安非他命调节转录肽；糖氧剥夺；内质网应激； 中图分类号：R363 Inhibitory effect of CART on OGD-induced endoplasmic reticulum stress in rat cortical neurons Qiu Bin1, Jia Zengqiang2, Liu Libing2, Wang Yachen1, Zhu Shigong1 (1. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191; 2. Weifang Medical University, Weifang, 261053) Abstract: Object: To investigate the effect of cocaine- and amphetamine-regulated transcript (CART) on oxygen and glucose deprivation (OGD)-induced endoplasmic reticulum stress in rat cortical neurons. Method: The primary cultured cortical neurons were established from embryonic rats (E18). The neuronal injury was induced by OGD method. The cell survival was analysed by MTT method, and the expression of endoplasmic reticulum stress (ER stress) markers (GRP78, CHOP and caspase12) were determined by western blotting. Results: Compared to Control group, neuronal survival was obviously decreased in OGD group (P 0.05), while expression of ER stress markers (GRP78, CHOP and caspase12) were significantly upregulated (P 0.05). In CART pretreatment group, cell survival was remarkably enhanced (P 0.05), while expression of GRP78, CHOP and caspase12 was siginicantly reduced (P 0.05) compared to OGD group. Conclusion: CART ameliorates OGD-induced injury by ER stress inhibition in rat cortical neurons. Key words: neuroprotection; CART; OGD; endoplasmic reticulum