鸡腿菇液体发酵工艺的优化.pdfVIP

安徽农业科学,Journal of Anhui Agri . Sci .2009 ,37( 15) :7179 - 7181,7186 责任编辑 孙 红忠 责任校对 卢瑶 鸡腿菇液体发酵工艺的优化 兰蓉, 杨国伟, 吴志明, 苑函 ( 北京 电子科技职业学院, 北京 100029) 摘要 [ 目的] 对鸡腿菇液体发酵培养基和培养条件进行筛选及优化 。[ 方法] 以鸡腿菇为供试材料, 胞外多糖和菌丝体生物量为指标, 通过单因素试验和正交试验对鸡腿菇液体发酵培养基配方及培养条件进行筛选及优化 。[ 结果] 鸡腿菇液体发酵产胞 外多糖 的最佳培 养基组合为: 葡萄糖2 .0 % , 酵母 膏0 .75 % ,CaCl 2 0 .2 % ; 鸡腿菇液体发酵产菌丝体 的最佳发酵培养基组合为: 葡萄糖2 % , 酵母 膏0 .75 % , KHPO 0 .2 % ; 鸡腿菇液体发酵产胞外多糖和菌丝体 的最佳发酵条件均为: 起始pH 值7 .0 , 接种量20 .0 % , 装液量 120 ml , 培养天数6 d 。 2 4 [ 结论] 该研 究为鸡腿菇的进一步开发提供 了科学依据 。 关键词 鸡腿菇; 液体发酵; 菌丝体; 胞外多糖 中图分类号 S188 文献标识码 A 文章编号 0517 - 6611(2009) 15 - 07179 - 03 Opti mization of Liquid Fer mentation Technol ogy for Coprinus co matua LAN Rong et al ( Beij ing Electronic Science and Technology Vocational College ,Beij ing 100029) Abstract [ Obj ective] The ai mwas to screen and opti mize the liquid fermentation mediumand culture condition of Copri nus co matua . [ Method] With C . comatua as tested material , extracellular polysaccharide and mycelial biomass as indexes , the liquid fermentation mediumformula and culture condition of C . comatua were screened and opti mized by single factor experi ment and orthogonal test . [ Result] The opti mal composition of mediums for C . co matua liquid fermentation producing extracellular polysaccharide were as follows :2 .0 % glucose , 0 .75 % yeast extract and 0 .2 % CaCl 2 , The opti mal composi- tion of fermentation mediums for C . comatua liquid fermentation producing mycelia were as follows :2 .0 % glucose , 0 .75 % yeast extract and 0 .2 % KHPO . The opti mal fer mentation conditions of C . comatua liquid fer mentation producing extracellular polysaccharide and mycelia were as follows : ini- 2 4 tial pHvalue of 7 .0 , inoculation amount of 20 .0 % , liquid volume of 120