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中国血吸虫病防治杂志 2009年第21卷第5期 ChinJSchi !!rU !!:三:!: : :
[文章编号] 1005-66612【009)05-0437-06 ·综述 ·
Application ofmoleculartechniquesindetection and
quantificationofhelminthicinfectioninmolluschost
W eiFu.rong,LvShan,ZhangYi
[Abstract] Moleculartechniquesraeimportantdetectionandquantificationmethodsinmodem lifescience.Inthispaper,thefeatures,
sensitivity,specificity,advantages,drawbacksandapplicationinhelminthofeachmolecularmethodraeintroduced·
[Keywords] Helminth;Mollusc;Moleculratechnique;Detection
}Correspondingauthor
[CLCnumber] R383 [Docmu entcode] A
Praasiticdiseasesarestillathreattohumanhealthandaccount polymerasechainreaction(PCR)amplificationandderivedtech‘
foraconsiderablefraction ofglobaldiseaseburden,especiallyin niques[ . Withthe enhancementofsensitivityand specificityof
ruralareasandsomepoorurbansettingsoflow·incomecountries… . molecularmeans.thesubjectivityisgreatlyreduced[4]_While,
Althoughsomecontrolprogramstragetingthesediseasesraeeffec— therearestillproblemswhich limittheirapplicationasstandardiza—
tiretoreducehumaninfections,therearenew challengestocontrol tionofdetection .Thedevelopmentandapplicationofmolecular
frame.Takingschistosomiasisofrexample,rgeatachievementhas detectiontoolsofrparasitesraeongoing.
beenmadethrough population—basedtreatmentprorgams,butthey 1 Parasitologicalandimmunologicaltechniques
mightnotdramatically reducepraasite transmission inhighlyen- Parasitologicaltechniquesbasedonmicroscopy,suchassnail
demicareas .Therefore,theWorld Health Organization recom— crushing,cercarialshedding,andthehistologicalmethod,arethe
mendsthatresearchshould
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