烟草中石油醚提取物测定方法改善.pdfVIP

中国烟草科学 2010 ，31 （1）：41-43 41 烟草中石油醚提取物测定方法改进 邵金良，黎其万，刘宏程，和丽忠，樊建麟，杜丽娟，束继红 （云南省农业科学院质量标准与检测技术研究所，昆明 650223 ） 摘 要：为提高烟草石油醚提取物含量的测定速度，进行了标准(YC/T 176-2003)测定方法的改进试验：①增加烟样浸泡过 夜预处理步骤；②称量介质由索氏提取器的接收瓶改为滤纸筒。试验结果表明，①增加烟样浸泡过夜预处理后，样品的提取 时间缩短2 h；②采用称量索氏提取器接收瓶计量，一套提取装置一次仅能提取1 个样品，改为称量滤纸筒计量后，一套提 取装置一次可提取 12 个样品，显著减少了提取设备、石油醚用量、电耗和水耗；③改进法的RSD 小于或略大于 1.0%；④ 改进法与标准法测定结果的相对偏差小于2.2% 。该法适合烟草石油醚提取物的快速测定。 关键词：烟草；石油醚提取物；测定 中图分类号：TS411 文章编号：1007-5119(2010)01-0041-03 DOI: 10.3969/j.issn.1007-5119.2010.01.011 Improvement on Total Petroleum Ether Extract Content Determination in Tobacco SHAO Jinliang, LI Qiwan, LIU Hongcheng, HE Lizhong, FAN Jianlin, DU Lijuan, SHU Jihong (Institute of Agriculture Quality standards＆Testing Technique, Yunnan Academy of Agricultural Science, Kunming 650223, China) Abstract: In order to quickly determine the content of total petroleum ether extract in tobacco, the standard method was improved, such as dipping the tobacco sample overnight, and adopting sieve papers canister instead of Soxhlet extractor in weighing. Results showed that the extract time was shortened by 2 hours after dipping the tobacco sample overnight. After adopting sieve papers canister, 12 samples were extracted once in a set of equipment, thus the new method could evidently save extract equipment, reduce the use of petroleum ether and the consumption of electricity and water, and accelerate determination speed. The relative standard deviation(RSD) of the improved method was below or slightly over 1.0%. The method was adequate for quick determination of total petroleum ether extract contents in tobacco. Keywords: tobacco; total petroleum ether extract; determination 石油醚提取物总量是衡量烟叶品质的一个重 1 材料与方法 要指标[1-2] 。目前，烟草石油醚提取