生防菌NH-BS-2000 (Bacillus subtilis) 对西瓜枯萎病的防治效果及其促长作用.docVIP

生防菌NH-BS-2000(Bacillus subtilis)对西瓜枯萎病的防治效果及其促长作用 朱育菁,葛慈斌,蓝江林,林抗美,肖荣凤[收稿日期:2007-04-10 基金项目:国家高技术研究发展计划(863计划)(2006AA10A211);福建省自然科学基金2006J0068;福建省发改委重点项目闽发改投资[2006]781号,福建省科技厅闽宁合作项目(2004Y034)。 作者简介:朱育菁(1972?),女,博士,主要从事植物病虫害生物防治研究。]* (福建省农科院生物技术研究所,福建 福州 350003) 摘 要:为了实现对西瓜枯萎病进行生物防治,研制出能替代化学农药的生物农药,本研究进行枯草芽孢杆菌NH-BS-2000对西瓜枯萎病的防治效果及促长作用的田间小区试验。试验结果表明:生防菌NH-BS-2000对西瓜苗期枯萎病的防治效果可达61.62%,高于化学药剂三环唑和维线克41.65%和37.55%,表现在能促进植株根部的生长。在处理30 d后,生防菌处理组对西瓜枯萎病的防治效果达83.14%,显著高于三环唑处理组和维线克的18.09%和25.53%,表明生防菌比化学农药具有更长的持效期,同时还能促进西瓜植株的株高、叶片数、着花数、座果数、叶长和叶宽等的生长。说明NH-BS-2000菌株具备作为枯萎病生防菌的良好优势,有望开发出瓜类枯萎病的生防菌剂。研究还发现生防菌NH-BS-2000的施用影响了土壤根际的微生物群落,显著提高了放线菌的含量。 关键词:生防菌NH-BS-2000;枯草芽孢杆菌;西瓜枯萎病;防治效果;促长作用 中图分类号:文献标识码: 文章编号: Control Efficacy of Biocontrol Strain NH-BS-2000 Bacillus subtilis against Wilt Disease of Watermelon and its Promoting Effect on the Growth ZHU Yu-jing, GE Ci-bin, LAN Jianglin, LIN Kanmei, XIAO Rong-feng, LIU Bo Institute of Biotechnology, Fujian Academy of Agricultural Sciences,Fuzhou 350003,China Abstract:The field experiment on the control efficacy of biocontrol strain NH-BS-2000 Bacillus subtilis against wilt disease of watermelon and its promotiong effect on the growth was conducted at Fuzhou, Fujian, China. The result showed that the control efficacy of NH-BS-2000 against the wilt disease of watermelon seedlings was 61.62%, higher than 41.65 nad 37.55% of chemical fungicide Tricyclazole and Weixianke, respectively. At 30 days after treatments, NH-BS-2000 had a control efficacy of 83.14%, which was significantly greater than 18.09% of Tricyclazole and 25.53% of Weixianke, and it also promted the development of plant height, leaf number, flower number, fruit number, leaf height and leaf width. The results indicated that NH-BS-2000 could be a good biocontrol strain for the development of a bio-fungicide against wilt disease of melon. Besides, the usage of NH-BS-2000 increased the number of actinomycete at the rhizospheric soils of watermelon seedlings. Keywords:Biocontro