“青大一号”紫花苜蓿叶蛋白提取研究.pdfVIP

ofAnhni 安徽农业科学，Journal Agi。Sci．2013，41(13)：5727—5730 责任编辑乔利利责任校对卢瑶 “青大一号紫花苜蓿叶蛋白提取研究 刘晓峰1，达哇卓玛1，杨国柱2(1．青海省玉树州曲麻莱县草原站，青海玉树815500；2。青海大学，青海西宁810000) 摘要[目的]探究“青大一号”紫花苜蓿叶蛋白的提取工艺。[方法]采用酸化加热法配合加盐法提取“青大一号”紫花苜蓿叶蛋白；以 单因素试验设计考察料水比、加盐量、pH、预浸时间、絮凝时间、絮凝温度6因素对苜蓿叶蛋白粗蛋白质提取率的影响；同时设计k(34) 正交试验来确定“青大一号”紫花苜蓿的苜蓿叶蛋白最佳提取条件。[结果]l‘青大一号”紫花苜蓿叶蛋白的最佳提取条件：絮凝温度为 70℃，料液比为1：10，加盐量为1％，絮凝时间为5 rnin，pH为4。[结论]为紫花苜蓿的工业化生产提供了理论依据。 关键词紫花苜蓿；叶蛋白；粗蛋白提取率；提取工艺 中图分类号$541+．1文献标识码A 文章编号0517—6611(2013)13—05727—04 onExtraction ofLeafProteinfrom saliva No．1 Study Technology MedicagoL．CY．Qingda LIU et Grassland 815500) Xiao-fengal(QumalaiStation，Yushu，Qinghai aimwasto theextraction ofleaf from sativa technology Medicago No．1．[Method] Abstract[Objective]111eexplore protein L．CV．Qingda six With sativa No．1asrawmaterial．alfalfaU)cwasextracted withsaltmethod。Theeffectsof MedicagoL．CV．Qingda byacid-heating single on factorsliketherateofmealto timeandflocculation theextraction water，saltconcentration，pH，immersiontime，flocculation temperature rateofLPCcrude werestudied ontheresultsof factor single single protein byusing experiment．Based expefi- meritwasconductedto the ofLPCextractionandthe conditionof LPCfrom s础西口var． study impact process optimum extraeting Medicago The conditionsforU)Cextractionwereas flocculation was flocculation OingdaNo．1．『Resultloptimum followed：the temperature70℃；the timewas5