石英砂负载过氧化物酶的制备及应用初探.pdfVIP

中国科技论文在线 石英砂负载过氧化物酶的制备及应用初探# 1 2* 陆隽鹤 ，谢卫平 （1. 南京农业大学资源与环境学院； 5 2. 江苏省宜兴市环境监测站） 摘要：本文研究以石英砂为载体，在其表面固定辣根过氧化物酶的方法。石英砂表面用壳聚 糖进行预处理，然后利用层层组装技术，通过静电吸引将酶层层负载到其表面。酶的固定化 回收率和活性回收率分别达到70%和78% 。固定化酶的最适pH 在4.5 左右，比游离酶略有 10 降低。固定化酶的热稳定性和储存稳定性较游离酶有明显提高。利用固定化酶可有效降解污 染土壤中的硝基苯，显示出在土壤污染修复方面的应用潜力。 关键词：环境保护工程；土壤修复；辣根过氧化物酶；固定化；层层组装技术；硝基苯 中图分类号：X53 15 Immobilization of horseradish peroxidase on quartz sands and its application in soil remediation 1 2 Lu Junhe , Xie Weiping (1. College of Resources and Environmental Sciences, Nanjing Agricultural University; 2. Yixing Environmental Monitoring Station of Jiangsu) 20 Abstract : In this paper a method of immobilization of horseradish peroxidase (HRP) onto quartz sands was developed. The sands were pretreated with chitosan and enzyme was loaded onto their surface thereafter via layer-by-layer technology with was based on electrastatic interaction. The immobilization recovery and activity recovery of HRP was 70% and 78%, respectively. The optimal pH for the immobilized enzyme was 4.5 which was lower than free enzyme. Thermo stability and longevity of 25 HRP were significantly imporved after immobilization. It was found the immobilized HRP could effectively degrade nitrobenzene in soil, which demonstrates its potential application in soil remediation. Key words: environmental protection engineering; soil remediation; horseradish peroxidase; immobilization; layer-