功能性低聚糖合成中糖基转移酶研究进展.pdfVIP

358 2013, Vol.34, No.09 食品科学 ※专题论述 功能性低聚糖合成中糖基转移酶研究进展 李 玉，路福平，王正祥 (工业发酵微生物教育部重点实验室，工业酶国家工程实验室，天津科技大学生物工程学院，天津 300457) 摘  要：低聚异麦芽糖、低聚果糖、岩藻糖基化寡糖和唾液酸化寡糖等功能性低聚糖因具有显著促双歧杆菌增殖的 α 作用,已被广泛应用于婴幼儿乳粉及食品中，目前合成这些功能糖的主要途径是利用微生物所产的 -葡萄糖基转移 酶、果糖基转移酶、岩藻糖基转移酶或唾液酸转移酶等糖基转移酶，以葡萄糖、蔗糖或乳糖等作为底物进行合成。 本文针对这几类微生物糖基转移酶的来源、性质、活性、表达和在功能性低聚糖合成中的应用以及相关研究进行简 要的概括和总结,为高活力糖基转移酶的研究与开发以及改善其在功能低聚糖生产中的应用性能提供一定的参考。 α 关键词： -葡萄糖基转移酶；果糖基转移酶；岩藻糖基转移酶；唾液酸转移酶 Recent Advance in Research and Application of Several Glycosyltransferases in the Synthesis of Functional Oligosaccharides LI Yu ，LU Fu-ping，WANG Zheng-xiang (Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, National Engineering Laboratory for Industrial Enzymes, College of Biotechnology, Tianj in University of Science and Technology, Tianj in 300457, China) Abstract ：Isomaltooligosacharide, fructooligosaccharides, fucosylated oligosaccharide and sialyloligosaccharide have been widely applied in milk powder for infants and young children because of its special physiological effect to promote the growth of bifidobacterium. These functional oligosaccharides are produced by α-glycosyltransferase, fructosyltransferase, fucosyltransferase and sialyltransferase, using glucose, sucrose and lactose as substrates, respectively . This paper reviews recent research advances in the microbial souces, properties, activity and expression of these enzymes and their applications in the synthesis of functional oligosaccharides in order to provide refereces for the development of highly active glycosyltransferases and the improvement of their applicability for the production of fun