慢性髓系白血病急变期差异表达基因的筛选.pdfVIP

·840 · 南方医科大学学报（J South Med Univ ） 2012;32(6) 临床研究 慢性髓系白血病急变期差异表达基因的筛选 骆许静，张进芳，刘晓力，杜庆锋，许 娜，许露露，黄彬涛，肖小珍 南方医科大学南方医院血液科，广东 广州 510515 摘要：目的筛选慢性髓系白血病急性变相关关键基因，探寻疾病进展机制及潜在的生物标志物。方法应用基因芯片技术筛选 与慢性髓系白血病急变期相关基因，采用生物信息学方法对这些差异表达基因进行基因本体分类，亚细胞定位以及组织选择性 分析，结合转录因子预测和网络谱图构建，找出差异表达基因中的关键分子并进行初步验证。结果得到一组定位于白细胞膜 的基因集，在整个调控网络中，CD40、CCR3、LGALS3、RGS3、CEACAM3 及转录因子RAC 1、CTNNB1、TP53、NF-kB 处于网络 节点位置，可能是疾病进展的关键调控基因，采用RT-PCR 方法初步证实CEACAM3、RGS3、CTNNB1和RAC 1在急变期患者表 达显著增高。结论CEACAM3、RGS3、CTNNB1和RAC 1可能是慢性髓系白血病由慢性期向急变期转变的关键基因和生物标 志物。 关键词：慢性髓系白血病；急变期；基因芯片；生物信息学 中图分类号：R733.72 文献标志码：A 文章编号：1673-4254（2012）06-0840-03 doi: 10.3969/j.issn. 1673-4254.2012.06.018 /kcms/detail/44.1627.R1747.013.html Identification of differentially expressed genes related to blastic crisis in chronic myeloid leukemia LUO Xujing, ZHANG Jinfang, LIU Xiaoli, DU Qingfeng, XU Na, XU Lulu, HUANG Bintao, XIAO Xiaozhen Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To identify differentially expressed genes between chronic phase and blast crisis in chronic myeloid leukemia, explore the mechanism and screen potential biomarkers of disease progression. Methods The differences in the gene expression profiles of bone marrow mononuclear cells between chronic phase and blastic crisis were examined using DNA microarray. PANTHER database, Genomatix database and Bibliosphere software were used to analyze and predict the critical genes or transcription factors during disease progression. Some of the genes or transcription factors were selected for verification by semi-quantitative RT-PCR. Results In blastic crisis, 68 of the 1176 tested genes were obviously up-regulated. Sixteen of these differential genes were selectively expressed