两种超氧化物歧化酶（SOD）活性测定方法的比较研究.pdfVIP

两种超氧化物歧化酶（SOD）活性测定方法的比较研究 牛丹丹，乔琰，刘杰，杨旭* (华中师范大学 生命科学学院环境科学实验室 武汉 430079) 摘 要 超氧化物歧化酶（SOD）是一种预防型抗氧化剂，广泛存在于各种生物中。它能清除链反应引 发阶段的超氧阴离子自由基（O2-. ），保护细胞免受损伤，对机体的氧化与抗氧化平衡起着至关重要的作用。 SOD活性测定的方法很多， 本研究通过对经相同染毒方法处理的小鼠不同器官进行SOD活性测定，从原理、 操作、结果的标准差及组间差别显著性等方面对NBT法和黄嘌呤氧化酶法这两种方法进行了比较，认为至少 在类似实验条件下，前者要比后者更为适合，是值得推荐的方法。 关键词 SOD SOD活性测定 甲醛 小鼠 氧化损伤 Comparative research of two methods for mensurating the activity of SOD NIU Dandan, QIAO Yan, LIU Jie, YANG Xu. (Lab. of Environmental Science, Central China Normal University, Wuhan 430079) Abstract: Superoxide dismutase (SOD) is a type of preventive antioxidant, which is widely existent in diverse organisms. It can eliminate O2-. during the induced phase of the chain reactions to protect the cells from damage, thus it plays an important role in the balance of the oxidation and antioxidation. There are many ways for mensurating the activity of SOD. By the measurement of SOD’s activity in different organs of mice treated with the same exposure of formaldehyde, we compared two methods of them from their principles, operations, the standard deviations and significant differences of the results. We believe that the former method is better than the latter one, at least in the similar experiment conditions, so is recommendable. Key words: SOD mensureation of the activity of SOD Formaldehyde Mice Oxidation damage 自由基是机体正常代谢的产物，在机体多种生理活动中是不可缺少的，如吞噬细胞的杀 菌作用，调节免疫等都与自由基密切相关。一旦由于某些原因（外伤、感染、衰老等）造成