实时荧光PCR法与培养法检测胆囊胆汁中细菌的比较.docVIP

实时荧光PCR法与培养法同时检测胆囊胆汁中细菌的比较 罗小兵1,2，郑培明1,2，乔铁*1,2，马瑞红1,2，罗振亮1,2，杨柳青1,2 广州市番禺区第二人民医院；2. 广州市番禺区胆囊病研究所。 通讯地址：广州市南沙区大岗镇兴业路7号，邮编：511470) 摘要 目的 比较实时荧光PCR法与培养法检测胆囊胆汁中细菌有无差别。方法 连续分析70例胆囊结石患者的胆囊胆汁，每例胆汁同时使用实时荧光 PCR法检测其中细菌16SrRNA基因和培养法(需氧+厌氧)检测有无细菌生长，对比二种方法的阳性率和符合情况。结果 培养法检出细菌阳性胆汁8例(细菌8株)，阳性率 11.4 %；培养法阳性的8例胆汁实时荧光PCR法同时阳性，而62例培养阴性胆汁则未出现阳性，二者符合率达到99.9%。结论 实时荧光PCR法与培养法检测胆囊胆汁中细菌有很高的符合率，但二者的检出率无差别。 关键词 实时荧光PCR；胆囊胆汁；细菌；培养；16SrRNA The analysis of Real-time Fluorescence PCR compared with the culture method to detect bacteria in the gallbladder bile LUO Xiao-bing 1,2, ZHENG Peiming1,2, QIAO Tie * 1,2, MA Rui-hong 1,2, LUO Zhen-liang 1,2, YANG Liu-qing 1,2 (1.The Second Peoples Hospital of Panyu District, Guangzhou; 2. Institute of gallbladder disease of Panyu District, Guangzhou. Address: No.7, Xingye Road, Dagang Town, Nansha District, Guangzhou, Post Code: 511470) Abstract Objective To compare the detection rate of the real-time PCR to detect bacteria in the gallbladder bile with the culture method. Methods Consecutive 70 cases of gallbladder bile from the patients with gallstones were investigated, each bile were detected for bacteria using real-time PCR which detect the presence of bacterial 16S rRNA genes and culture (aerobic + anaerobic) simultaneously, then compared to the positive rate and coincidence of the two methods . Results Bacteria were detected using culture method in 8 cases of bile (8 strains of bacteria), the positive rate of 11.4%; these 8 culture-positive biles were positive simultaneously with Real-time PCR method, and 62 cases of culture-negative bile were also negative, the coincidence rate of 99.9% were for two methods. Conclusion There is a high coincidence rate and the same detection rate of Real-time PCR and the culture method to detect bacteria in the gallbladder bile. Keywords Real-time fluorescence PCR; Gallbladder bile; Bacteria; Culture; 16SrRNA 胆囊结石是一种多发病，其患者常合并急慢性胆囊炎，引起多种不良反应和临床症状。对胆囊结石患者的胆囊胆汁进行细菌检测，有助于区分炎症类型、指导术后合理用药和病因学研究。目前，PCR检测细菌16S rRNA 基因已较多