基于壳聚糖.pdfVIP

280 2011, Vol. 32, No. 04 食品科学 ※技术应用 汤俊琪，庞广昌 * ，梁新义，冯苍松 (天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院，天津 300134) 在玻碳电极表面修饰壳聚糖和纳米金，以纳米金对抗体等生物分子的良好亲和性，固定抗 - αs- 酪蛋白(anti- αs- 酪蛋白)抗体制得酪蛋白免疫传感器。通过循环伏安法考察电极表面的电化学特性，并对该免疫传感器的性能 进行研究。该免疫传感器对以磷酸盐缓冲液溶解的 αs- 酪蛋白进行检测。响应电流与酪蛋白抗原质量浓度的对数在 1～10000ng/mL 的范围内线性相关。该免疫传感器制作简单，成本较低，操作方便，可应用于牛奶质量和非乳蛋 白掺杂使假的检测。 免疫传感器；壳聚糖；纳米金；酪蛋白；计时电流法；生物传感器；玻碳电极；循环伏安法 Development of Casein Immunosensor Modified by Chitosan and Nano-Gold TANG Jun-qi ，PANG Guang-chang* ，LIANG Xin-yi ，FENG Cang-song (Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China) Abstract：A novel casein immunosensor for detecting αs-casein was developed through the modification of chitosan and nano- gold on the glassy carbon electrode. The modification procedure was electrochemically monitored by cyclic voltammetry to explore the characteristics of the electrode surface and the performance of the immunosensor. The developed immunosensor was used to detect αs-casein in phosphate buffer. An excellent linear range between response current and αs-casein concentration was observed in the concentration range of 1 －10000 ng/mL. Therefore, this developed immunosensor is characteristics of simple preparation, low cost, convenient operation, and can be applied to the safety detection of milk. Key words ：immunosensor ；chitosan ；nano-gold ；casein ；chronoamperometry ；biosensor ；glassy carbon electrode ； cyclic voltammetry 中图分类号：TS201 ；O657.32 文献标识码：B 文章编号：1002-6630(2011)04-0280-04 壳聚糖(chitosan ，CHIT) 是天然高分子聚合物甲壳 其生物活性，在生物传感器的制作中被普遍使用[5-7] 。对 素的脱乙酰基产物，价格低廉，来源广泛